TY - JOUR
T1 - Genetic and molecular characterization of Candystripe1 transposition events in sorghum
AU - Carvalho, Carlos H.S.
AU - Boddu, Jayanand
AU - Zehr, Usha B.
AU - Axtell, John D.
AU - Pedersen, Jeffery F.
AU - Chopra, Surinder
N1 - Funding Information:
This article is dedicated to the memory of late Prof. John D. Axtell, whose efforts led to the identification of Candystripe1 induced mutations. We are thankful to Dr Thomas Peterson, Iowa State University for constructive ideas during the development of this research project. We thank Ms Catherine Svabek for her excellent technical assistance, and Drs Dan Knievel and David Huff for their valuable suggestions and review of this manuscript. Research support to SC was provided under the Hatch projects 3855 and 3905, and National Research Initiative of the USDA Cooperative State Research, Education and Extension Service Grant Number 2002-35318-12676.
PY - 2005/7
Y1 - 2005/7
N2 - In sorghum, the Candystripe1 (Cs1) transposable element causes a variegated pericarp phenotype due to its excision activity from the y1 (yellow seed1) locus. The Y1 is a transcription regulator which is required for the biosynthesis of red 3-deoxyflavonoid pigments. Somatic variability in the transposition behavior of Cs1 was observed via biochemical analysis of 3-deoxyflavonoids in the leaf tissues of the Y1-cs alleles. Using somatic excisions of Cs1 as a tool, we establish that the Cs1 is active in young seedlings and the y1 locus is also functional in these tissues. Several somatic and germinal excision events were characterized and sequence analysis of independent events predominantly showed 2-bp footprints. Further, with the goal of understanding the properties of Cs1 that would facilitate the development of a transposon tagging system in sorghum, germinal excisions of Cs1 from y1 were used as a marker. Transposition of Cs1 was followed by characterization of putative insertion events. Genetic linkage between mutant phenotypes and the co-segregating restriction fragments of Cs1 provided additional evidence that Cs1 is an active transposable element in sorghum.
AB - In sorghum, the Candystripe1 (Cs1) transposable element causes a variegated pericarp phenotype due to its excision activity from the y1 (yellow seed1) locus. The Y1 is a transcription regulator which is required for the biosynthesis of red 3-deoxyflavonoid pigments. Somatic variability in the transposition behavior of Cs1 was observed via biochemical analysis of 3-deoxyflavonoids in the leaf tissues of the Y1-cs alleles. Using somatic excisions of Cs1 as a tool, we establish that the Cs1 is active in young seedlings and the y1 locus is also functional in these tissues. Several somatic and germinal excision events were characterized and sequence analysis of independent events predominantly showed 2-bp footprints. Further, with the goal of understanding the properties of Cs1 that would facilitate the development of a transposon tagging system in sorghum, germinal excisions of Cs1 from y1 were used as a marker. Transposition of Cs1 was followed by characterization of putative insertion events. Genetic linkage between mutant phenotypes and the co-segregating restriction fragments of Cs1 provided additional evidence that Cs1 is an active transposable element in sorghum.
UR - http://www.scopus.com/inward/record.url?scp=21744445918&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21744445918&partnerID=8YFLogxK
U2 - 10.1007/s10709-005-2268-8
DO - 10.1007/s10709-005-2268-8
M3 - Article
C2 - 16134333
AN - SCOPUS:21744445918
SN - 0016-6707
VL - 124
SP - 201
EP - 212
JO - Genetica
JF - Genetica
IS - 2
ER -