TY - JOUR
T1 - Genetic bottlenecks reduce population variation in an experimental RNA virus population
AU - Li, Hongye
AU - Roossinck, Marilyn J.
PY - 2004/10
Y1 - 2004/10
N2 - Genetic bottlenecks are stochastic events that limit genetic variation in a population and result in founding populations that can lead to genetic drift. Evidence of past genetic bottlenecks in numerous biological systems, from mammals to viruses, has been described. In this study, we used an artificial population of Cucumber mosaic virus consisting of 12 restriction enzyme marker-bearing mutants. This population was inoculated onto young leaves of tobacco plants and monitored throughout the course of systemic infection. We show here that the genetic variation in a defined population of an RNA virus is significantly, stochastically, and reproducibly reduced during the systemic infection process, providing clear evidence of a genetic bottleneck.
AB - Genetic bottlenecks are stochastic events that limit genetic variation in a population and result in founding populations that can lead to genetic drift. Evidence of past genetic bottlenecks in numerous biological systems, from mammals to viruses, has been described. In this study, we used an artificial population of Cucumber mosaic virus consisting of 12 restriction enzyme marker-bearing mutants. This population was inoculated onto young leaves of tobacco plants and monitored throughout the course of systemic infection. We show here that the genetic variation in a defined population of an RNA virus is significantly, stochastically, and reproducibly reduced during the systemic infection process, providing clear evidence of a genetic bottleneck.
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U2 - 10.1128/JVI.78.19.10582-10587.2004
DO - 10.1128/JVI.78.19.10582-10587.2004
M3 - Article
C2 - 15367625
AN - SCOPUS:4544331687
SN - 0022-538X
VL - 78
SP - 10582
EP - 10587
JO - Journal of virology
JF - Journal of virology
IS - 19
ER -