TY - JOUR
T1 - Genetic linkage map construction and QTL identification of juvenile growth traits in Torreya grandis
AU - Zeng, Yanru
AU - Ye, Shengyue
AU - Yu, Weiwu
AU - Wu, Song
AU - Hou, Wei
AU - Wu, Rongling
AU - Dai, Wensheng
AU - Chang, Jun
N1 - Funding Information:
Funding Publication of this work was supported by grants (2008C22003 and 2012C12904-12) from Department of Science and Technology, Zhejiang Province, China, NSF/IOS-0923975 and "863" Program 2013AA102605 of China.
Funding Information:
Publication of this work was supported by grants (2008C22003 and 2012C12904-12) from Department of Science and Technology, Zhejiang Province, China, NSF/ IOS-0923975 and “863” Program 2013AA102605 of China.
Publisher Copyright:
© 2014 Zeng et al.
PY - 2014
Y1 - 2014
N2 - Torreya grandis Fort. ex Lindl, a conifer species widely distributed in Southeastern China, is of high economic value by producing edible, nutrient seeds. However, knowledge about the genome structure and organization of this species is poorly understood, thereby limiting the effective use of its gene resources. Here, we report on a first genetic linkage map for Torreya grandis using 96 progeny randomly chosen from a half-sib family of a commercially cultivated variety of this species, Torreya grandis Fort. ex Lindl cv. Merrillii. The map contains 262 molecular markers, i.e., 75 random amplified polymorphic DNAs (RAPD), 119 inter-simple sequence repeats (ISSR) and 62 amplified fragments length polymorphisms (AFLP), and spans a total of 7,139.9 cM, separated by 10 linkage groups. The linkage map was used to map quantitative trait loci (QTLs) associated with juvenile growth traits by functional mapping. We identified four basal diameter-related QTLs on linkage groups 1, 5 and 9; four heightrelated QTLs on linkage groups 1, 2, 5 and 8. It was observed that the genetic effects of QTLs on growth traits vary with age, suggesting the dynamic behavior of growth QTLs. Part of the QTLs was found to display a pleiotropic effect on basal diameter growth and height growth.
AB - Torreya grandis Fort. ex Lindl, a conifer species widely distributed in Southeastern China, is of high economic value by producing edible, nutrient seeds. However, knowledge about the genome structure and organization of this species is poorly understood, thereby limiting the effective use of its gene resources. Here, we report on a first genetic linkage map for Torreya grandis using 96 progeny randomly chosen from a half-sib family of a commercially cultivated variety of this species, Torreya grandis Fort. ex Lindl cv. Merrillii. The map contains 262 molecular markers, i.e., 75 random amplified polymorphic DNAs (RAPD), 119 inter-simple sequence repeats (ISSR) and 62 amplified fragments length polymorphisms (AFLP), and spans a total of 7,139.9 cM, separated by 10 linkage groups. The linkage map was used to map quantitative trait loci (QTLs) associated with juvenile growth traits by functional mapping. We identified four basal diameter-related QTLs on linkage groups 1, 5 and 9; four heightrelated QTLs on linkage groups 1, 2, 5 and 8. It was observed that the genetic effects of QTLs on growth traits vary with age, suggesting the dynamic behavior of growth QTLs. Part of the QTLs was found to display a pleiotropic effect on basal diameter growth and height growth.
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U2 - 10.1186/1471-2156-15-S1-S2
DO - 10.1186/1471-2156-15-S1-S2
M3 - Article
C2 - 25079139
AN - SCOPUS:84988905018
SN - 1471-2156
VL - 15
JO - BMC Genetics
JF - BMC Genetics
M1 - S2
ER -