TY - JOUR
T1 - Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata
AU - Wang, Yan
AU - Wang, Xi
AU - McCubbin, Andrew G.
AU - Kao, Teh Hui
N1 - Funding Information:
We thank Marcy Uyenoyama (Duke University) for valuable comments, the Cornell Research Foundation and Carol Hamilton for providing BIBAC2 vector, Christiane Gebhardt (Max Planck Institute for Plant Breeding Research, Cologne, Germany) for providing CP100, and Ed Newbigin (University of Melbourne, Melbourne, Australia) for providing 48A. We also thank Anthony H. Omeis for the greenhouse work and Jiong Wang for routine lab assistance. This work was supported by grants from the National Science Foundation (grants IBN-9982659 and IBN-0235176 to T.-h.K).
PY - 2003/11
Y1 - 2003/11
N2 - Gametophytic self-incompatibility (SI) possessed by the Solanaceae is controlled by a highly polymorphic locus called the S locus. The S locus contains two linked genes, S-RNase, which determines female specificity, and the as yet unidentified pollen S gene, which determines male specificity in SI interactions. To identify the pollen S gene of Petunia inflata, we had previously used mRNA differential display and subtractive hybridization to identify 13 pollen-expressed genes that showed S-haplotype-specific RFLP. Here, we carried out recombination analysis of 1205 F2 plants to determine the genetic distance between each of these S-linked genes and S-RNase. Recombination was observed between four of the genes (3.16, G211, G212, and G221) and S-RNase, whereas no recombination was observed for the other nine genes (3.2, 3.15, A113, A134, A181, A301, G261, X9, and X11). A genetic map of the S locus was constructed, with 3.16 and G221 delimiting the outer limits. None of the observed crossovers disrupted SI, suggesting that all the genes required for SI are contained in the chromosomal region defined by 3.16 and G221. These results and our preliminary chromosome walking results suggest that the S locus is a huge multigene complex. Allelic sequence diversity of G221 and 3.16, as well as of 3.2, 3.15, A113, A134 and G261, was determined by comparing two or three alleles of their cDNA and/or genomic sequences. In contrast to S-RNase, all these genes showed very low degrees of allelic sequence diversity in the coding regions, introns, and flanking regions.
AB - Gametophytic self-incompatibility (SI) possessed by the Solanaceae is controlled by a highly polymorphic locus called the S locus. The S locus contains two linked genes, S-RNase, which determines female specificity, and the as yet unidentified pollen S gene, which determines male specificity in SI interactions. To identify the pollen S gene of Petunia inflata, we had previously used mRNA differential display and subtractive hybridization to identify 13 pollen-expressed genes that showed S-haplotype-specific RFLP. Here, we carried out recombination analysis of 1205 F2 plants to determine the genetic distance between each of these S-linked genes and S-RNase. Recombination was observed between four of the genes (3.16, G211, G212, and G221) and S-RNase, whereas no recombination was observed for the other nine genes (3.2, 3.15, A113, A134, A181, A301, G261, X9, and X11). A genetic map of the S locus was constructed, with 3.16 and G221 delimiting the outer limits. None of the observed crossovers disrupted SI, suggesting that all the genes required for SI are contained in the chromosomal region defined by 3.16 and G221. These results and our preliminary chromosome walking results suggest that the S locus is a huge multigene complex. Allelic sequence diversity of G221 and 3.16, as well as of 3.2, 3.15, A113, A134 and G261, was determined by comparing two or three alleles of their cDNA and/or genomic sequences. In contrast to S-RNase, all these genes showed very low degrees of allelic sequence diversity in the coding regions, introns, and flanking regions.
UR - http://www.scopus.com/inward/record.url?scp=1542580431&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1542580431&partnerID=8YFLogxK
U2 - 10.1023/B:PLAN.0000019068.00034.09
DO - 10.1023/B:PLAN.0000019068.00034.09
M3 - Article
C2 - 15010619
AN - SCOPUS:1542580431
SN - 0167-4412
VL - 53
SP - 565
EP - 580
JO - Plant molecular biology
JF - Plant molecular biology
IS - 4
ER -