TY - JOUR
T1 - Genome wide association mapping of epi-cuticular wax genes in Sorghum bicolor
AU - Elango, Dinakaran
AU - Xue, Weiya
AU - Chopra, Surinder
N1 - Funding Information:
We thank GRIN and Dr. Wilfred Vermerris (University of Florida) for providing the sorghum mapping panels SAP and MNC respectively. We thank Qixian Tan for sample collection, wax extraction and data recording. We thank Dr. Gregory Wayne Roth for his advice during field experiments. We acknowledge the assistance of Jin Cui, Debamalya Chatterjee, Iffa Gaffoor, Kameron Wittmeyer and Bin Liu for field sample collection and phenotyping. We thank Scott Harkcom, Penn State Agronomy farm manager for facilitating field preparation and assistance with crop management.
Funding Information:
This work was supported by a USDA/NIFA Awards 2011-67009-30017, 2019-70006-30442, and AES Awards PEN04430 and PEN04613 to SC. Acknowledgements
Publisher Copyright:
© 2020, Prof. H.S. Srivastava Foundation for Science and Society.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Sorghum accumulates epi-cuticular wax (EW) in leaves, sheaths, and culms. EW reduces the transpirational and nontranspirational (nonstomatal) water loss and protects the plant from severe drought stress in addition to imparting resistance against insect pests. Results presented here are from the analysis of EW content of 387 diverse sorghum accessions and its genome-wide association study (GWAS). EW content in sorghum leaves ranged from 0.1 to 29.7 mg cm−2 with a mean value of 5.1 mg cm−2. GWAS using 265,487 single nucleotide polymorphisms identified thirty-seven putative genes associated (P < 9.89E−06) with EW biosynthesis and transport in sorghum. Major EW biosynthetic genes identified included 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, an Ankyrin repeat protein, a bHLH-MYC, and an R2R3-MYB transcription factor. Genes involved in EW regulation or transport included an ABC transporter, a Lipid exporter ABCA1, a Multidrug resistance protein, Inositol 1, 3, 4-trisphosphate 5/6-kinase, and a Cytochrome P450. This GWA study thus demonstrates the potential for genetic manipulation of EW content in sorghum for better adaptation to biotic and abiotic stress.
AB - Sorghum accumulates epi-cuticular wax (EW) in leaves, sheaths, and culms. EW reduces the transpirational and nontranspirational (nonstomatal) water loss and protects the plant from severe drought stress in addition to imparting resistance against insect pests. Results presented here are from the analysis of EW content of 387 diverse sorghum accessions and its genome-wide association study (GWAS). EW content in sorghum leaves ranged from 0.1 to 29.7 mg cm−2 with a mean value of 5.1 mg cm−2. GWAS using 265,487 single nucleotide polymorphisms identified thirty-seven putative genes associated (P < 9.89E−06) with EW biosynthesis and transport in sorghum. Major EW biosynthetic genes identified included 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, an Ankyrin repeat protein, a bHLH-MYC, and an R2R3-MYB transcription factor. Genes involved in EW regulation or transport included an ABC transporter, a Lipid exporter ABCA1, a Multidrug resistance protein, Inositol 1, 3, 4-trisphosphate 5/6-kinase, and a Cytochrome P450. This GWA study thus demonstrates the potential for genetic manipulation of EW content in sorghum for better adaptation to biotic and abiotic stress.
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U2 - 10.1007/s12298-020-00848-5
DO - 10.1007/s12298-020-00848-5
M3 - Article
C2 - 32801499
AN - SCOPUS:85087975041
SN - 0971-5894
VL - 26
SP - 1727
EP - 1737
JO - Physiology and Molecular Biology of Plants
JF - Physiology and Molecular Biology of Plants
IS - 8
ER -