Genome-wide associations and transcriptional profiling reveal ROS regulation as one underlying mechanism of sheath blight resistance in rice

Eula Gems Oreiro, Earlyn Kate Grimares, Genelou Atienza-Grande, Ian Lorenzo Quibod, Veronica Roman-Reyna, Ricardo Oliva

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Rice sheath blight, caused by the necrotrophic fungus Rhizoctonia solani Kühn, continues to be an important and challenging rice disease worldwide. Here, we used genome-wide association studies over a high-density rice array to facilitate the identification of potential novel genes and quantitative trait loci related to sheath blight resistance. We identified multiple regions that significantly associated with independent disease components in chromosomes 1, 4, and 11 under controlled condition. In particular, we investigated qLN1128, a quantitative trait locus enriched with defense-related genes that reduce disease lesions in a near-isogenic line. RNA profiling of the line carrying qLN1128 showed a number of differentially expressed genes related to the reactive oxygen species (ROS)-redox pathway. Histochemical staining revealed less ROS accumulation on the resistant line, suggesting efficient ROS deregulation that delays pathogen colonization. The detection of genomic regions controlling multiple mechanisms of resistance to sheath blight will provide tools to design effective breeding interventions in rice.

Original languageEnglish (US)
Pages (from-to)212-222
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume33
Issue number2
DOIs
StatePublished - 2020

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this