Genomic characterization of a turkey reovirus field strain by Next-Generation Sequencing

Yi Tang; Huaguang Lu; Aswathy Sebastian; Yin Ting Yeh; Craig A. Praul; Istvan U. Albert; Si Yang Zheng

doi:10.1016/j.meegid.2015.03.029

Genomic characterization of a turkey reovirus field strain by Next-Generation Sequencing

Yi Tang, Huaguang Lu, Aswathy Sebastian, Yin Ting Yeh, Craig A. Praul, Istvan U. Albert, Si Yang Zheng

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Abstract

The genome of a turkey arthritis reovirus (TARV) field strain (Reo/PA/Turkey/22342/13), isolated from a turkey flock in Pennsylvania (PA) in 2013, has been sequenced using Next-Generation Sequencing (NGS) on the Illumina MiSeq platform. The genome of the PA TARV field strain was 23,496. bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192. bp (S4) to 3959. bp (L1). The 5' and 3' conserved terminal sequences of the PA TARV field strain were similar to the two Minnesota (MN) TARVs (MN9 and MN10) published recently and avian orthoreovirus (ARV) reference strains. Phylogenetic analysis of the nucleotide sequences of all 10 genome segments revealed that there was a low to significant nucleotide sequence divergence between the PA TARV field strain and reference TARV and ARV strains. Analysis of the PA TARV sequence indicates that this PA TARV field strain is a unique strain and is different from the TARV MN9 or MN10 in M2 segment genes and ARV S1133 vaccine strain.

Original language	English (US)
Pages (from-to)	313-321
Number of pages	9
Journal	Infection, Genetics and Evolution
Volume	32
DOIs	https://doi.org/10.1016/j.meegid.2015.03.029
State	Published - Jun 1 2015

All Science Journal Classification (ASJC) codes

Microbiology
Ecology, Evolution, Behavior and Systematics
Molecular Biology
Genetics
Microbiology (medical)
Infectious Diseases

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.meegid.2015.03.029

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@article{29800058c5c6402583975462e93552d6,

title = "Genomic characterization of a turkey reovirus field strain by Next-Generation Sequencing",

abstract = "The genome of a turkey arthritis reovirus (TARV) field strain (Reo/PA/Turkey/22342/13), isolated from a turkey flock in Pennsylvania (PA) in 2013, has been sequenced using Next-Generation Sequencing (NGS) on the Illumina MiSeq platform. The genome of the PA TARV field strain was 23,496. bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192. bp (S4) to 3959. bp (L1). The 5' and 3' conserved terminal sequences of the PA TARV field strain were similar to the two Minnesota (MN) TARVs (MN9 and MN10) published recently and avian orthoreovirus (ARV) reference strains. Phylogenetic analysis of the nucleotide sequences of all 10 genome segments revealed that there was a low to significant nucleotide sequence divergence between the PA TARV field strain and reference TARV and ARV strains. Analysis of the PA TARV sequence indicates that this PA TARV field strain is a unique strain and is different from the TARV MN9 or MN10 in M2 segment genes and ARV S1133 vaccine strain.",

author = "Yi Tang and Huaguang Lu and Aswathy Sebastian and Yeh, {Yin Ting} and Praul, {Craig A.} and Albert, {Istvan U.} and Zheng, {Si Yang}",

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doi = "10.1016/j.meegid.2015.03.029",

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AU - Tang, Yi

AU - Lu, Huaguang

AU - Sebastian, Aswathy

AU - Yeh, Yin Ting

AU - Praul, Craig A.

AU - Albert, Istvan U.

AU - Zheng, Si Yang

PY - 2015/6/1

Y1 - 2015/6/1

N2 - The genome of a turkey arthritis reovirus (TARV) field strain (Reo/PA/Turkey/22342/13), isolated from a turkey flock in Pennsylvania (PA) in 2013, has been sequenced using Next-Generation Sequencing (NGS) on the Illumina MiSeq platform. The genome of the PA TARV field strain was 23,496. bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192. bp (S4) to 3959. bp (L1). The 5' and 3' conserved terminal sequences of the PA TARV field strain were similar to the two Minnesota (MN) TARVs (MN9 and MN10) published recently and avian orthoreovirus (ARV) reference strains. Phylogenetic analysis of the nucleotide sequences of all 10 genome segments revealed that there was a low to significant nucleotide sequence divergence between the PA TARV field strain and reference TARV and ARV strains. Analysis of the PA TARV sequence indicates that this PA TARV field strain is a unique strain and is different from the TARV MN9 or MN10 in M2 segment genes and ARV S1133 vaccine strain.

AB - The genome of a turkey arthritis reovirus (TARV) field strain (Reo/PA/Turkey/22342/13), isolated from a turkey flock in Pennsylvania (PA) in 2013, has been sequenced using Next-Generation Sequencing (NGS) on the Illumina MiSeq platform. The genome of the PA TARV field strain was 23,496. bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192. bp (S4) to 3959. bp (L1). The 5' and 3' conserved terminal sequences of the PA TARV field strain were similar to the two Minnesota (MN) TARVs (MN9 and MN10) published recently and avian orthoreovirus (ARV) reference strains. Phylogenetic analysis of the nucleotide sequences of all 10 genome segments revealed that there was a low to significant nucleotide sequence divergence between the PA TARV field strain and reference TARV and ARV strains. Analysis of the PA TARV sequence indicates that this PA TARV field strain is a unique strain and is different from the TARV MN9 or MN10 in M2 segment genes and ARV S1133 vaccine strain.

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Genomic characterization of a turkey reovirus field strain by Next-Generation Sequencing

Abstract

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