TY - JOUR
T1 - Genomic characterization of velogenic avian orthoavulavirus 1 isolates from poultry workers
T2 - Implications to emergence and its zoonotic potential towards public health
AU - Shabbir, Muhammad Zubair
AU - Nissly, Ruth Helmus
AU - Ahad, Abdul
AU - Rabbani, Masood
AU - Lim, Levina
AU - Chothe, Shubhada K.
AU - Subbiah, Murugan
AU - Sebastian, Aswathy
AU - Albert, Istvan
AU - Ul-Rahman, Aziz
AU - Jayarao, Bhushan M.
AU - Kuchipudi, Suresh V.
N1 - Funding Information:
This work was supported by the startup research grant of the Pennsylvania State University (S.V.K.). The authors wish to thank Craig Praul and the Penn State Genomics Core Facility, University Park, PA for useful advice and guidance for planning the whole genome sequencing.
Publisher Copyright:
© 2021 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2021/2/1
Y1 - 2021/2/1
N2 - Objective: To carry out the genetic characterization and evolutionary analysis of three avian orthoavulavirus 1 (AOAV-1) isolates from poultry workers with respiratory symptoms. Methods: Using Illumina MiSeq, whole-genome sequencing was carried out to assess the evolutionary dynamics of three AOAV-1 isolates. A phylogenetic and comparative analysis of all coding genes was done using bioinformatics tools. Results: Phylogenetic analysis and genetic distance estimation suggested a close relationship among human- and avian-originated velogenic strains of genotype XIII, sub-genotype XIII.2.1. Several substitutions in the significant structural and biological motifs were exclusively identified in the human-originated strains. Conclusions: To our knowledge, this is the first report of a velogenic AOAV-1 isolate from natural infection of the human upper respiratory tract. Our findings highlight the evolution and zoonotic potential of velogenic AOAV-1 in a disease endemic setting.
AB - Objective: To carry out the genetic characterization and evolutionary analysis of three avian orthoavulavirus 1 (AOAV-1) isolates from poultry workers with respiratory symptoms. Methods: Using Illumina MiSeq, whole-genome sequencing was carried out to assess the evolutionary dynamics of three AOAV-1 isolates. A phylogenetic and comparative analysis of all coding genes was done using bioinformatics tools. Results: Phylogenetic analysis and genetic distance estimation suggested a close relationship among human- and avian-originated velogenic strains of genotype XIII, sub-genotype XIII.2.1. Several substitutions in the significant structural and biological motifs were exclusively identified in the human-originated strains. Conclusions: To our knowledge, this is the first report of a velogenic AOAV-1 isolate from natural infection of the human upper respiratory tract. Our findings highlight the evolution and zoonotic potential of velogenic AOAV-1 in a disease endemic setting.
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U2 - 10.4103/1995-7645.306762
DO - 10.4103/1995-7645.306762
M3 - Article
AN - SCOPUS:85100024665
SN - 1995-7645
VL - 14
SP - 64
EP - 72
JO - Asian Pacific Journal of Tropical Medicine
JF - Asian Pacific Journal of Tropical Medicine
IS - 2
ER -