TY - JOUR
T1 - Genomic Deoxyribonucleic Acid Restriction Fragment Length Polymorphism of Streptococcus uberis
T2 - Evidence of Clonal Diversity
AU - Jayarao, B. M.
AU - Schilling, E. E.
AU - Oliver, S. P.
N1 - Funding Information:
This investigation was supported by a Professional Development Award from The University of Tennessee, the Tennessee Agricultural Experiment Station, and The University of Tennessee College of Veterinary
PY - 1993
Y1 - 1993
N2 - Fifty plasmid-free strains of Streptococcus uberis, including 40 strains isolated from cows in two dairy herds in Tennessee with subclinical and clinical mastitis, 9 strains isolated from cows in the US, and 1 strain from Great Britain were examined for genomic DNA restriction fragment length polymorphisms. The 50 strains belonged to 35 DNA fingerprint patterns. Within a herd, fingerprint patterns from isolates obtained from cows with subclinical mastitis at different periods of the lactation cycle and from episodes of clinical mastitis were similar. Restriction fragment length polymorphism analysis revealed the presence of DNA fragments ≥21 kb in all 12 strains of S. uberis isolated from episodes of clinical mastitis and in only 8 of the 38 (21%) remaining strains. Cluster analysis placed the 50 strains into eight distinct clusters. Most strains from the northeastern US were confined to one cluster, whereas most of the isolates from episodes of clinical mastitis belonged to two different clusters. These data suggest that clonal diversity exists among strains of S. uberis isolated from bovine mammary secretions.
AB - Fifty plasmid-free strains of Streptococcus uberis, including 40 strains isolated from cows in two dairy herds in Tennessee with subclinical and clinical mastitis, 9 strains isolated from cows in the US, and 1 strain from Great Britain were examined for genomic DNA restriction fragment length polymorphisms. The 50 strains belonged to 35 DNA fingerprint patterns. Within a herd, fingerprint patterns from isolates obtained from cows with subclinical mastitis at different periods of the lactation cycle and from episodes of clinical mastitis were similar. Restriction fragment length polymorphism analysis revealed the presence of DNA fragments ≥21 kb in all 12 strains of S. uberis isolated from episodes of clinical mastitis and in only 8 of the 38 (21%) remaining strains. Cluster analysis placed the 50 strains into eight distinct clusters. Most strains from the northeastern US were confined to one cluster, whereas most of the isolates from episodes of clinical mastitis belonged to two different clusters. These data suggest that clonal diversity exists among strains of S. uberis isolated from bovine mammary secretions.
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U2 - 10.3168/jds.S0022-0302(93)77367-1
DO - 10.3168/jds.S0022-0302(93)77367-1
M3 - Article
C2 - 8095273
AN - SCOPUS:0027551224
SN - 0022-0302
VL - 76
SP - 468
EP - 474
JO - Journal of dairy science
JF - Journal of dairy science
IS - 2
ER -