Genomic homologous recombination in planta

Susannah Gal, Barbara Pisan, Thomas Hohn, Nigel Grimsley, Barbara Hohn

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42 Scopus citations


A system for monitoring intrachromosomal homologous combination in whole plants is described. A multimer of cauliflower mosaic virus (CaMV) sequences, arranged such that CaMV could only be produced by recombination, was integrated into Brassica napus nuclear DNA. This set-up allowed scoring of recombination events by the appearance of viral symptoms. The repeated homologous regions were derived from two different strains of CaMV so that diffrent recombinant viruses (i.e. different recombination events) could be distinguished. In most of the transgenic plants, a single major virus species was detected. About half of the transgenic plants contained viruses of the same type, suggesting a hotspot for recombination. The remainder of the plants contained viruses with cross-over sites distributed throughout the rest of the homologous sequence. Sequence analysis of two recombinant molecules suggests that mismatch repair is linked to the recombination process.

Original languageEnglish (US)
Pages (from-to)1571-1578
Number of pages8
JournalEMBO Journal
Issue number6
StatePublished - 1991

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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