TY - JOUR
T1 - Genomic identification of intergeneric hybrids in New World wood-warblers (Aves: Parulidae)
AU - Toews, David P.L.
AU - Kramer, Gunnar R.
AU - Jones, Andrew W.
AU - Brennan, Courtney L.
AU - Cloud, Benjamin E.
AU - Andersen, David E.
AU - Lovette, Irby J.
AU - Streby, Henry
N1 - Funding Information:
We thank Bronwyn Butcher for assistance in the laboratory and Hattie Saloka for providing logistical support. Molecular laboratory work at the Cleveland Museum of Natural History was supported by the William A. and Nancy R. Klamm Endowment. Funding for the analyses was provided by Pennsylvania State University and the Cornell Lab of Ornithology. The Wisconsin Department of Natural Resources and the US Geological Survey Bird Banding Laboratory provided permits for capturing and sampling blood from Vermivora warblers in Wisconsin. We thank the Louisiana State University Museum of Natural Science Collection of Genetic Resources for loan of the cerulean warbler tissue samples. We thank Robert Zink, Lan-Nhi Phung and two anonymous reviewers for providing comments on a previous version of this manuscript. Any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the US Government or the University of Minnesota.
Funding Information:
We thank Bronwyn Butcher for assistance in the laboratory and Hattie Saloka for providing logistical support. Molecular laboratory work at the Cleveland Museum of Natural History was supported by the William A. and Nancy R. Klamm Endowment. Funding for the analyses was provided by Pennsylvania State University and the Cornell Lab of Ornithology. The Wisconsin Department of Natural Resources and the US Geological Survey Bird Banding Laboratory provided permits for capturing and sampling blood from Vermivora warblers in Wisconsin. We thank the Louisiana State University Museum of Natural Science Collection of Genetic Resources for loan of the cerulean warbler tissue samples. We thank Robert Zink, Lan-Nhi Phùng and two anonymous reviewers for providing comments on a previous version of this manuscript. Any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the US Government or the University of Minnesota.
Publisher Copyright:
© 2020 The Linnean Society of London, Biological Journal of the Linnean Society.
PY - 2020/8/26
Y1 - 2020/8/26
N2 - The documentation of hybrids between distantly related taxa can illustrate an initial step to explain how genes might move between species that do not exhibit complete reproductive isolation. In birds, some of the most phylogenetically distant hybrid combinations occur between genera. Traditionally, morphological and plumage characters have been used to assign the identity of the parental species of a putative hybrid, although recently, nuclear introns also have been used. Here, we demonstrate how high-throughput short-read DNA sequence data can be used to identify the parentage of a putative intergeneric hybrid, in this case between a blue-winged warbler (Vermivora cyanoptera) and a cerulean warbler (Setophaga cerulea). This hybrid had mitochondrial DNA of a cerulean warbler, indicating the maternal parent. For hundreds of single nucleotide polymorphisms within six regions of the nuclear genome that differentiate blue-winged warblers and golden-winged warblers (Vermivora chrysoptera), the hybrid had roughly equal ancestry assignment to blue-winged and cerulean warblers, suggesting a blue-winged warbler as the paternal parent species and demonstrating that this was a first generation (F1) hybrid between these species. Unlike other recently characterized intergeneric warbler hybrids, this individual hybrid learned to song match its maternal parent species, suggesting that it might have been the result of an extra-pair mating and raised in a cerulean warbler nest.
AB - The documentation of hybrids between distantly related taxa can illustrate an initial step to explain how genes might move between species that do not exhibit complete reproductive isolation. In birds, some of the most phylogenetically distant hybrid combinations occur between genera. Traditionally, morphological and plumage characters have been used to assign the identity of the parental species of a putative hybrid, although recently, nuclear introns also have been used. Here, we demonstrate how high-throughput short-read DNA sequence data can be used to identify the parentage of a putative intergeneric hybrid, in this case between a blue-winged warbler (Vermivora cyanoptera) and a cerulean warbler (Setophaga cerulea). This hybrid had mitochondrial DNA of a cerulean warbler, indicating the maternal parent. For hundreds of single nucleotide polymorphisms within six regions of the nuclear genome that differentiate blue-winged warblers and golden-winged warblers (Vermivora chrysoptera), the hybrid had roughly equal ancestry assignment to blue-winged and cerulean warblers, suggesting a blue-winged warbler as the paternal parent species and demonstrating that this was a first generation (F1) hybrid between these species. Unlike other recently characterized intergeneric warbler hybrids, this individual hybrid learned to song match its maternal parent species, suggesting that it might have been the result of an extra-pair mating and raised in a cerulean warbler nest.
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U2 - 10.1093/biolinnean/blaa085
DO - 10.1093/biolinnean/blaa085
M3 - Article
AN - SCOPUS:85094811333
SN - 0024-4066
VL - 131
SP - 183
EP - 191
JO - Biological Journal of the Linnean Society
JF - Biological Journal of the Linnean Society
IS - 1
ER -