Genotypic characterization and phylogenetic relations of Pseudomonas sp. (formerly P. stutzeri) OX1

Francesca Radice; Viviana Orlandi; Valentina Massa; Lucia Cavalca; Antonella Demarta; Thomas K. Wood; Paola Barbieri

doi:10.1007/s00284-005-0355-9

Genotypic characterization and phylogenetic relations of Pseudomonas sp. (formerly P. stutzeri) OX1

Francesca Radice, Viviana Orlandi, Valentina Massa, Lucia Cavalca, Antonella Demarta, Thomas K. Wood, Paola Barbieri

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Abstract

Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment length polymorphism (MERFLP) analysis suggested that Pseudomonas sp. OX1 could not be classified as P. stutzeri. Phylogenetic analyses based on 16S rRNA and gyrB genes further confirmed that this strain belongs to the Pseudomonas (sensu stricto) genus, but not to the stutzeri species. The data obtained demonstrated that Pseudomonas sp. OX1 belongs to intrageneric cluster II and is related to the P. fluorescens-P. syringae complex.

Original language	English (US)
Pages (from-to)	395-399
Number of pages	5
Journal	Current Microbiology
Volume	52
Issue number	5
DOIs	https://doi.org/10.1007/s00284-005-0355-9
State	Published - May 2006

All Science Journal Classification (ASJC) codes

Microbiology
Applied Microbiology and Biotechnology

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title = "Genotypic characterization and phylogenetic relations of Pseudomonas sp. (formerly P. stutzeri) OX1",

abstract = "Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment length polymorphism (MERFLP) analysis suggested that Pseudomonas sp. OX1 could not be classified as P. stutzeri. Phylogenetic analyses based on 16S rRNA and gyrB genes further confirmed that this strain belongs to the Pseudomonas (sensu stricto) genus, but not to the stutzeri species. The data obtained demonstrated that Pseudomonas sp. OX1 belongs to intrageneric cluster II and is related to the P. fluorescens-P. syringae complex.",

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T1 - Genotypic characterization and phylogenetic relations of Pseudomonas sp. (formerly P. stutzeri) OX1

AU - Radice, Francesca

AU - Orlandi, Viviana

AU - Massa, Valentina

AU - Cavalca, Lucia

AU - Demarta, Antonella

AU - Wood, Thomas K.

AU - Barbieri, Paola

PY - 2006/5

Y1 - 2006/5

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AB - Pseudomonas sp. OX1, an aromatic compound-degrading bacterium that was tentatively identified by conventional biochemical methods as P. stutzeri, has now been investigated at the molecular level to clarify its taxonomic position. Amplified ribosomal DNA restriction analysis and multiple enzyme restriction fragment length polymorphism (MERFLP) analysis suggested that Pseudomonas sp. OX1 could not be classified as P. stutzeri. Phylogenetic analyses based on 16S rRNA and gyrB genes further confirmed that this strain belongs to the Pseudomonas (sensu stricto) genus, but not to the stutzeri species. The data obtained demonstrated that Pseudomonas sp. OX1 belongs to intrageneric cluster II and is related to the P. fluorescens-P. syringae complex.

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Genotypic characterization and phylogenetic relations of Pseudomonas sp. (formerly P. stutzeri) OX1

Abstract

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