TY - JOUR
T1 - Ghrelin Receptor Stimulation of the Lateral Parabrachial Nucleus in Rats Increases Food Intake but not Food Motivation
AU - Bake, Tina
AU - Le May, Marie V.
AU - Edvardsson, Christian E.
AU - Vogel, Heike
AU - Bergström, Ulrika
AU - Albers, Marjorie Nicholson
AU - Skibicka, Karolina P.
AU - Farkas, Imre
AU - Liposits, Zsolt
AU - Dickson, Suzanne L.
N1 - Funding Information:
This research was supported by the EC FP7 project “Nudge‐it” (grant agreement number 607310), the Swedish Research Council for Medicine (Vetenskapsrådet, grant number 2016‐02195; 2019‐01051), Hjärnfonden (FO2017‐0180; FO2018‐0262; FO2019‐0086), NovoNordisk Fonden (NNF17OC0027206; NNF19OC0056694), National Research, Development and Innovation Office (K128278), and Avtal om Läkarutbildning och Forskning (ALFGBG‐723681). The funding bodies did not have a role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the article for publication.
Publisher Copyright:
2020 The Authors. Obesity published by Wiley Periodicals LLC on behalf of The Obesity Society (TOS).
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Objective: The lateral parabrachial nucleus (lPBN) in the brainstem has emerged as a key area involved in feeding control that is targeted by several circulating anorexigenic hormones. Here, the objective was to determine whether the lPBN is also a relevant site for the orexigenic hormone ghrelin, inspired by studies in mice and rats showing that there is an abundance of ghrelin receptors in this area. Methods: This study first explored whether iPBN cells respond to ghrelin involving Fos mapping and electrophysiological studies in rats. Next, rats were injected acutely with ghrelin, a ghrelin receptor antagonist, or vehicle into the lPBN to investigate feeding-linked behaviors. Results: Curiously, ghrelin injection (intracerebroventricular or intravenous) increased Fos protein expression in the lPBN yet the predominant electrophysiological response was inhibitory. Intra-lPBN ghrelin injection increased chow or high-fat diet intake, whereas the antagonist decreased chow intake only. In a choice paradigm, intra-lPBN ghrelin increased intake of chow but not lard or sucrose. Intra-lPBN ghrelin did not alter progressive ratio lever pressing for sucrose or conditioned place preference for chocolate. Conclusions: The lPBN is a novel locus from which ghrelin can alter consummatory behaviors (food intake and choice) but not appetitive behaviors (food reward and motivation).
AB - Objective: The lateral parabrachial nucleus (lPBN) in the brainstem has emerged as a key area involved in feeding control that is targeted by several circulating anorexigenic hormones. Here, the objective was to determine whether the lPBN is also a relevant site for the orexigenic hormone ghrelin, inspired by studies in mice and rats showing that there is an abundance of ghrelin receptors in this area. Methods: This study first explored whether iPBN cells respond to ghrelin involving Fos mapping and electrophysiological studies in rats. Next, rats were injected acutely with ghrelin, a ghrelin receptor antagonist, or vehicle into the lPBN to investigate feeding-linked behaviors. Results: Curiously, ghrelin injection (intracerebroventricular or intravenous) increased Fos protein expression in the lPBN yet the predominant electrophysiological response was inhibitory. Intra-lPBN ghrelin injection increased chow or high-fat diet intake, whereas the antagonist decreased chow intake only. In a choice paradigm, intra-lPBN ghrelin increased intake of chow but not lard or sucrose. Intra-lPBN ghrelin did not alter progressive ratio lever pressing for sucrose or conditioned place preference for chocolate. Conclusions: The lPBN is a novel locus from which ghrelin can alter consummatory behaviors (food intake and choice) but not appetitive behaviors (food reward and motivation).
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U2 - 10.1002/oby.22875
DO - 10.1002/oby.22875
M3 - Article
C2 - 32627950
AN - SCOPUS:85087477277
SN - 1930-7381
VL - 28
SP - 1503
EP - 1511
JO - Obesity
JF - Obesity
IS - 8
ER -