Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film

Hong Wu; Jun Wang; Xinhuang Kang; Chongmin Wang; Donghai Wang; Jun Liu; Ilhan A. Aksay; Yuehe Lin

doi:10.1016/j.talanta.2009.06.054

Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film

Hong Wu, Jun Wang, Xinhuang Kang, Chongmin Wang, Donghai Wang, Jun Liu, Ilhan A. Aksay, Yuehe Lin

Research output: Contribution to journal › Article › peer-review

419 Scopus citations

Abstract

The bionanocomposite film consisting of glucose oxidase/Pt/functional graphene sheets/chitosan (GOD/Pt/FGS/chitosan) for glucose sensing is described. With the electrocatalytic synergy of FGS and Pt nanoparticles to hydrogen peroxide, a sensitive biosensor with a detection limit of 0.6 μM glucose was achieved. The biosensor also has good reproducibility, long-term stability and negligible interfering signals from ascorbic acid and uric acid comparing with the response to glucose. The large surface area and good electrical conductivity of graphene suggests that graphene is a potential candidate as a sensor material. The hybrid nanocomposite glucose sensor provides new opportunity for clinical diagnosis and point-of-care applications.

Original language	English (US)
Pages (from-to)	403-406
Number of pages	4
Journal	Talanta
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/j.talanta.2009.06.054
State	Published - Nov 15 2009

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1016/j.talanta.2009.06.054

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title = "Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film",

abstract = "The bionanocomposite film consisting of glucose oxidase/Pt/functional graphene sheets/chitosan (GOD/Pt/FGS/chitosan) for glucose sensing is described. With the electrocatalytic synergy of FGS and Pt nanoparticles to hydrogen peroxide, a sensitive biosensor with a detection limit of 0.6 μM glucose was achieved. The biosensor also has good reproducibility, long-term stability and negligible interfering signals from ascorbic acid and uric acid comparing with the response to glucose. The large surface area and good electrical conductivity of graphene suggests that graphene is a potential candidate as a sensor material. The hybrid nanocomposite glucose sensor provides new opportunity for clinical diagnosis and point-of-care applications.",

author = "Hong Wu and Jun Wang and Xinhuang Kang and Chongmin Wang and Donghai Wang and Jun Liu and Aksay, {Ilhan A.} and Yuehe Lin",

note = "Funding Information: This work was supported by a laboratory-directed research and development program at Pacific Northwest National Laboratory (PNNL). The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) and located at PNNL. PNNL is operated by Battelle for DOE under Contract DE-AC05-76RL01830. Ilhan A. Aksay acknowledges support from Army Research Office (ARO)/Multidisciplinary Research Initiative (MURI) under grant number W911NF-04-1-0170 and the Directed Technologies, Inc.",

year = "2009",

month = nov,

day = "15",

doi = "10.1016/j.talanta.2009.06.054",

language = "English (US)",

volume = "80",

pages = "403--406",

journal = "Talanta",

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T1 - Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film

AU - Wu, Hong

AU - Wang, Jun

AU - Kang, Xinhuang

AU - Wang, Chongmin

AU - Wang, Donghai

AU - Liu, Jun

AU - Aksay, Ilhan A.

AU - Lin, Yuehe

N1 - Funding Information: This work was supported by a laboratory-directed research and development program at Pacific Northwest National Laboratory (PNNL). The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) and located at PNNL. PNNL is operated by Battelle for DOE under Contract DE-AC05-76RL01830. Ilhan A. Aksay acknowledges support from Army Research Office (ARO)/Multidisciplinary Research Initiative (MURI) under grant number W911NF-04-1-0170 and the Directed Technologies, Inc.

PY - 2009/11/15

Y1 - 2009/11/15

N2 - The bionanocomposite film consisting of glucose oxidase/Pt/functional graphene sheets/chitosan (GOD/Pt/FGS/chitosan) for glucose sensing is described. With the electrocatalytic synergy of FGS and Pt nanoparticles to hydrogen peroxide, a sensitive biosensor with a detection limit of 0.6 μM glucose was achieved. The biosensor also has good reproducibility, long-term stability and negligible interfering signals from ascorbic acid and uric acid comparing with the response to glucose. The large surface area and good electrical conductivity of graphene suggests that graphene is a potential candidate as a sensor material. The hybrid nanocomposite glucose sensor provides new opportunity for clinical diagnosis and point-of-care applications.

AB - The bionanocomposite film consisting of glucose oxidase/Pt/functional graphene sheets/chitosan (GOD/Pt/FGS/chitosan) for glucose sensing is described. With the electrocatalytic synergy of FGS and Pt nanoparticles to hydrogen peroxide, a sensitive biosensor with a detection limit of 0.6 μM glucose was achieved. The biosensor also has good reproducibility, long-term stability and negligible interfering signals from ascorbic acid and uric acid comparing with the response to glucose. The large surface area and good electrical conductivity of graphene suggests that graphene is a potential candidate as a sensor material. The hybrid nanocomposite glucose sensor provides new opportunity for clinical diagnosis and point-of-care applications.

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SN - 0039-9140

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JO - Talanta

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ER -

Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film

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