TY - JOUR
T1 - Glucose decreases steady state mRNA content of hydrophobic surfactant proteins B and C in fetal rat lung explants
AU - Rayani, Hamid H.
AU - Gewolb, Ira H.
AU - Floros, Joanna
N1 - Funding Information:
Received 2 January 199; a8ccepted 25 May 19.98 The authors thank Janet O’Brien, John J. Wert Jr., and Gina Deiter frotechnical assistance and Dr. Sharon Xie frobiosattistical consultation. The work was supported by the National Institutes of Health, RO1 HL 388( J8F2) abny thedAmerican Diabetes Foundation ( IHG) . Address corresonpdence to Joanna Floros, PhD, Departmnt ofeCellular and Molecular Physiology, The Pensylvnania State University College of Medicine, 50U0niversity Drive, Hershey, PA 107, 3US3.A E-mail : [email protected]
PY - 1999
Y1 - 1999
N2 - The streptozotocin-induced diabetic (STZ-DB) rat model is associated with fetal hyperglycemia, but with low to normal plasma insulin concentration. Because surfactant protein (SP) mRNA content in fetal rat lung is decreased in STZ-DB pregnancy, we investigated the effect of increasing concentrations of glucose on SP gene expression in lung organ cultures. SP mRNA content (SP-A, SP-B, SP-C) was assessed by Northern blot analysis in fetal day 20 lung explants (term = 22 days) cultured for 44 hours in medium containing 10, 25, 50, or 100 mM glucose. Our findings were (1) No consistent alteration in SP-A mRNA content was observed at different glucose concentrations (P > .05); (2) SP-B and SP-C mRNA content were reduced in a dose-dependent manner when glucose concentration was increased from 10 mM to 100 mM. The mRNA content, compared to 10 mM glucose, decreased to 50-60% at 25 mM glucose, to 20-25% at 50 mM glucose, and to lower than 10% at 100 mM glucose (P < .01). These findings indicate that the decrease in SP-B and SP- C mRNA in fetuses of STZ-DB rats may be, in part, due to a direct effect of hyperglycemia, whereas the decrease in SP-A mRNA content in STZ-DB rats appears to be due to other effects of diabetes in pregnancy.
AB - The streptozotocin-induced diabetic (STZ-DB) rat model is associated with fetal hyperglycemia, but with low to normal plasma insulin concentration. Because surfactant protein (SP) mRNA content in fetal rat lung is decreased in STZ-DB pregnancy, we investigated the effect of increasing concentrations of glucose on SP gene expression in lung organ cultures. SP mRNA content (SP-A, SP-B, SP-C) was assessed by Northern blot analysis in fetal day 20 lung explants (term = 22 days) cultured for 44 hours in medium containing 10, 25, 50, or 100 mM glucose. Our findings were (1) No consistent alteration in SP-A mRNA content was observed at different glucose concentrations (P > .05); (2) SP-B and SP-C mRNA content were reduced in a dose-dependent manner when glucose concentration was increased from 10 mM to 100 mM. The mRNA content, compared to 10 mM glucose, decreased to 50-60% at 25 mM glucose, to 20-25% at 50 mM glucose, and to lower than 10% at 100 mM glucose (P < .01). These findings indicate that the decrease in SP-B and SP- C mRNA in fetuses of STZ-DB rats may be, in part, due to a direct effect of hyperglycemia, whereas the decrease in SP-A mRNA content in STZ-DB rats appears to be due to other effects of diabetes in pregnancy.
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U2 - 10.1080/019021499270439
DO - 10.1080/019021499270439
M3 - Article
C2 - 10027080
AN - SCOPUS:0033031057
SN - 0190-2148
VL - 25
SP - 69
EP - 79
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 1
ER -
