Glucose regulates transcription in yeast through a network of signaling pathways

Shadia Zaman, Soyeon I. Lippman, Lisa Schneper, Noam Slonim, James R. Broach

Research output: Contribution to journalArticlepeer-review

172 Scopus citations


Addition of glucose to yeast cells increases their growth rate and results in a massive restructuring of their transcriptional output. We have used microarray analysis in conjunction with conditional mutations to obtain a systems view of the signaling network responsible for glucose-induced transcriptional changes. We found that several well-studied signaling pathways - such as Snf1 and Rgt - are responsible for specialized but limited responses to glucose. However, 90% of the glucose-induced changes can be recapitulated by the activation of protein kinase A (PKA) or by the induction of PKB (Sch9). Blocking signaling through Sch9 does not interfere with the glucose response, whereas blocking signaling through PKA does. We conclude that both Sch9 and PKA regulate a massive, nutrient-responsive transcriptional program promoting growth, but that they do so in response to different nutritional inputs. Moreover, activating PKA completely recapitulates the transcriptional growth program in the absence of any increase in growth or metabolism, demonstrating that activation of the growth program results solely from the cell's perception of its nutritional status.

Original languageEnglish (US)
Article number245
JournalMolecular Systems Biology
StatePublished - Jan 20 2009

All Science Journal Classification (ASJC) codes

  • Information Systems
  • General Immunology and Microbiology
  • Applied Mathematics
  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • Computational Theory and Mathematics


Dive into the research topics of 'Glucose regulates transcription in yeast through a network of signaling pathways'. Together they form a unique fingerprint.

Cite this