TY - JOUR
T1 - Glycolipid based cubic nanoparticles
T2 - Preparation and structural aspects
AU - Abraham, Thomas
AU - Hato, Masakatsu
AU - Hirai, Mitsuhiro
N1 - Funding Information:
T.A. thankful to the Japan Society for Promotion of Science for JSPS award and the full financial support for this research work. The authors are grateful to Drs. D. Kato, D. Negishi, and Y. Abe at Lion Corporation, Process Development Research Center, for providing UPA 150 Particle Size Analyzer facility for the particle size measurements. Synchrotron SAXD was performed under the approval of the Photon Factory Program Advisory Committee (Proposal Nos. 2001G359 and 2003G137).
PY - 2004/5/15
Y1 - 2004/5/15
N2 - Kinetically stable cubic colloidal particle dispersion was produced from a glycolipid using a novel preparation strategy based on the dialysis principle. The use of synchrotron small-angle X-ray diffraction (SSAXD) permitted the identification of exact structure of these dispersed particles in the colloidal state. Dynamic light scattering methods were used to obtain size and size distributions. A glycoside, 1-O-phytanyl-β-D-xyloside (β-XP), that exhibits Pn3m cubic phase in an excess aqueous medium, was used as the lipid material. The dialysis technique includes controlled stirring action both inside and outside of the dialysis membrane tube. Initially, a mixed micellar system composed of β-XP, n-octyl-β-D-glucopyranoside (β-OG) and a triblock copolymer, Pluronic F127 (PL) was prepared in the aqueous medium. About 10 wt.% of PL to lipid weight was found to be sufficient to produce stable colloidal dispersions. The mean volume diameter of these colloidal particles was found to be in the range of 0.85±0.05 μm. The cubic phase structure of these colloidal particles is greatly depended on the final β-OG concentration level in the system. Coexistence of Im3m and Pn3m cubic structures has been identified in these colloidal particles. This coexistence has the characteristics of Bonnet relation, which forms a compelling case for the infinite periodic minimal surface (IPMS) descriptions. These colloidal particles could restore pure Pn3m phase structure, but a longer dialysis time was needed. This work, in general, will open up new possibilities for membrane protein reconstitution and other relevant biological applications using colloidal cubic lipid particles.
AB - Kinetically stable cubic colloidal particle dispersion was produced from a glycolipid using a novel preparation strategy based on the dialysis principle. The use of synchrotron small-angle X-ray diffraction (SSAXD) permitted the identification of exact structure of these dispersed particles in the colloidal state. Dynamic light scattering methods were used to obtain size and size distributions. A glycoside, 1-O-phytanyl-β-D-xyloside (β-XP), that exhibits Pn3m cubic phase in an excess aqueous medium, was used as the lipid material. The dialysis technique includes controlled stirring action both inside and outside of the dialysis membrane tube. Initially, a mixed micellar system composed of β-XP, n-octyl-β-D-glucopyranoside (β-OG) and a triblock copolymer, Pluronic F127 (PL) was prepared in the aqueous medium. About 10 wt.% of PL to lipid weight was found to be sufficient to produce stable colloidal dispersions. The mean volume diameter of these colloidal particles was found to be in the range of 0.85±0.05 μm. The cubic phase structure of these colloidal particles is greatly depended on the final β-OG concentration level in the system. Coexistence of Im3m and Pn3m cubic structures has been identified in these colloidal particles. This coexistence has the characteristics of Bonnet relation, which forms a compelling case for the infinite periodic minimal surface (IPMS) descriptions. These colloidal particles could restore pure Pn3m phase structure, but a longer dialysis time was needed. This work, in general, will open up new possibilities for membrane protein reconstitution and other relevant biological applications using colloidal cubic lipid particles.
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U2 - 10.1016/j.colsurfb.2004.02.015
DO - 10.1016/j.colsurfb.2004.02.015
M3 - Article
C2 - 15261043
AN - SCOPUS:2342536905
SN - 0927-7765
VL - 35
SP - 107
EP - 118
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 2
ER -