Glycolipids as receptors for Bacillus thuringienses crystal toxin

Joel S. Griffitts; Stuart M. Haslam; Tinglu Yang; Stephan F. Garczynski; Barbara Mulloy; Howard Morris; Paul S. Cremer; Anne Dell; Michael J. Adang; Raffi V. Aroian

doi:10.1126/science.1104444

Glycolipids as receptors for Bacillus thuringienses crystal toxin

Joel S. Griffitts, Stuart M. Haslam, Tinglu Yang, Stephan F. Garczynski, Barbara Mulloy, Howard Morris, Paul S. Cremer, Anne Dell, Michael J. Adang, Raffi V. Aroian

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304 Scopus citations

Abstract

The development of pest resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins used in transgenic and organic farming. Here, we demonstrate that (i) the major mechanism for Bt toxin resistance in Caenorhabditis elegans entails a loss of glycolipid carbohydrates; (ii) Bt toxin directly and specifically binds glycolipids; and (iii) this binding is carbohydrate-dependent and relevant for toxin action in vivo. These carbohydrates contain the arthroseries core conserved in insects and nematodes but lacking in vertebrates. We present evidence that insect glycolipids are also receptors for Bt toxin.

Original language	English (US)
Pages (from-to)	922-925
Number of pages	4
Journal	Science
Volume	307
Issue number	5711
DOIs	https://doi.org/10.1126/science.1104444
State	Published - Feb 11 2005

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title = "Glycolipids as receptors for Bacillus thuringienses crystal toxin",

abstract = "The development of pest resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins used in transgenic and organic farming. Here, we demonstrate that (i) the major mechanism for Bt toxin resistance in Caenorhabditis elegans entails a loss of glycolipid carbohydrates; (ii) Bt toxin directly and specifically binds glycolipids; and (iii) this binding is carbohydrate-dependent and relevant for toxin action in vivo. These carbohydrates contain the arthroseries core conserved in insects and nematodes but lacking in vertebrates. We present evidence that insect glycolipids are also receptors for Bt toxin.",

author = "Griffitts, {Joel S.} and Haslam, {Stuart M.} and Tinglu Yang and Garczynski, {Stephan F.} and Barbara Mulloy and Howard Morris and Cremer, {Paul S.} and Anne Dell and Adang, {Michael J.} and Aroian, {Raffi V.}",

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AU - Griffitts, Joel S.

AU - Haslam, Stuart M.

AU - Yang, Tinglu

AU - Garczynski, Stephan F.

AU - Mulloy, Barbara

AU - Morris, Howard

AU - Cremer, Paul S.

AU - Dell, Anne

AU - Adang, Michael J.

AU - Aroian, Raffi V.

PY - 2005/2/11

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AB - The development of pest resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins used in transgenic and organic farming. Here, we demonstrate that (i) the major mechanism for Bt toxin resistance in Caenorhabditis elegans entails a loss of glycolipid carbohydrates; (ii) Bt toxin directly and specifically binds glycolipids; and (iii) this binding is carbohydrate-dependent and relevant for toxin action in vivo. These carbohydrates contain the arthroseries core conserved in insects and nematodes but lacking in vertebrates. We present evidence that insect glycolipids are also receptors for Bt toxin.

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Glycolipids as receptors for Bacillus thuringienses crystal toxin

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