Background: GPI-anchored molecule-like protein (GML) has been identified as a new target molecule of p53. In esophageal cancer cell lines, expression of GML mRNA, which was found to be significantly correlated with in vitro anti-cancer drug sensitivity, was observed only in the presence of wild-type p53. Materials and Methods: The relationship between expression of GML mRNA and p53 gene status in 24 non-small cell lung cancer (NSCLC) cell lines was examined using RT-PCR and PCR-SSCP followed by direct DNA sequencing. Results: Expression of GML mRNA was observed in only 3 of 24 cell lines. In contrast to previous studies showing p53-dependent GML expression, of the 3 cell lines expressing GML mRNA, one had a p53 gene mutation (codon 245: Gly to Cys). Conclusion: These findings suggest that the expression of GML mRNA is infrequent and regulated in a p53-independent manner in NSCLC.
|Original language||English (US)|
|Number of pages||5|
|Issue number||5 B|
|State||Published - 1999|
All Science Journal Classification (ASJC) codes
- Cancer Research