Abstract
Green fluorescent protein (GFP) is widely used as an excellent reporter molecule in biochemistry and cell biology. Some biochemical and immunological assays require high-purity GFP. However, the majority of current procedures for GFP purification include multiple time-consuming chromatography steps with a low yield of the desired product or require tag-containing proteins. An alternative method is described for the GFP purification without affinity extensions using organic extraction yielding a highly homogeneous protein indistinguishable in spectroscopic properties from that purified by previous methods.
Original language | English (US) |
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Pages (from-to) | 382-386 |
Number of pages | 5 |
Journal | Protein Expression and Purification |
Volume | 14 |
Issue number | 3 |
DOIs | |
State | Published - Dec 1998 |
All Science Journal Classification (ASJC) codes
- Biotechnology