Abstract
Mammalian spermine synthase activity was strongly inhibited by S-methyl-5′-methylthioadenosine. This nucleoside was readily taken up by SV-3T3 cells and blocked the synthesis of spermine by these cells. However, there was a corresponding increase in spermidine content and there was no effect on cell growth. These results indicate that S-methyl-5′-methylthioadenosine should be a useful compound to evaluate the role of spermine in mammalian cell physiology and that in at least one cell line the synthesis of spermine is not required for a normal growth rate.
Original language | English (US) |
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Pages (from-to) | 82-89 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 133 |
Issue number | 1 |
DOIs | |
State | Published - Nov 27 1985 |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology