Growth of mammalian cells in the absence of the accumulation of spermine

Anthony E. Pegg, James K. Coward

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Mammalian spermine synthase activity was strongly inhibited by S-methyl-5′-methylthioadenosine. This nucleoside was readily taken up by SV-3T3 cells and blocked the synthesis of spermine by these cells. However, there was a corresponding increase in spermidine content and there was no effect on cell growth. These results indicate that S-methyl-5′-methylthioadenosine should be a useful compound to evaluate the role of spermine in mammalian cell physiology and that in at least one cell line the synthesis of spermine is not required for a normal growth rate.

Original languageEnglish (US)
Pages (from-to)82-89
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Nov 27 1985

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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