Abstract
World Health Organization’s aim to eliminate malaria from developing/resource-limited economies requires easy access to low-cost, highly sensitive, and specific screening. We present a handheld nucleic acid testing device with on-chip automated sample preparation to detect malaria (Plasmodium falciparum) infection from a whole blood sample as a feasibility study. We used a simple two-reagent-based purification-free protocol to prepare the whole blood sample on a piezo pump pressure-driven microfluidic cartridge. The cartridge includes a unique mixing chamber for sample preparation and metering structures to dispense a predetermined volume of the sample lysate mixture into four chambers containing a reaction mix. The parasite genomic DNA concentration can be estimated by monitoring the fluorescence generated from the loop-mediated isothermal amplification reaction in real time. We achieved a sensitivity of ∼0.42 parasite/μL of whole blood, sufficient for detecting asymptomatic malaria parasite carriers.
Original language | English (US) |
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Pages (from-to) | 673-683 |
Number of pages | 11 |
Journal | ACS Sensors |
Volume | 8 |
Issue number | 2 |
DOIs | |
State | Published - Feb 24 2023 |
All Science Journal Classification (ASJC) codes
- Bioengineering
- Instrumentation
- Process Chemistry and Technology
- Fluid Flow and Transfer Processes