Haptoglobin typing from fatty tissues after extraction with non-ionic detergents

Reena Roy

doi:10.1016/0379-0738(91)90176-J

Haptoglobin typing from fatty tissues after extraction with non-ionic detergents

Reena Roy

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Of the seven non-ionic detergents tested, Span 40^®, Tween 20^® and Triton X-45^® were suitable for extraction of haptoglobin (Hp) from fatty tissues. The extracted samples were subjected to electrophoresis and subsequently stained with o-tolidine. Enzyme-immunoassay and Western blotting (electrophoretic) techniques were used successfully to determine the Hp phenotypes from minute quantities of tissue samples after extractions with non-ionic detergents.

Original language	English (US)
Pages (from-to)	95-102
Number of pages	8
Journal	Forensic Science International
Volume	49
Issue number	1
DOIs	https://doi.org/10.1016/0379-0738(91)90176-J
State	Published - Jan 1 1991

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine

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10.1016/0379-0738(91)90176-J

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title = "Haptoglobin typing from fatty tissues after extraction with non-ionic detergents",

abstract = "Of the seven non-ionic detergents tested, Span 40{\textregistered}, Tween 20{\textregistered} and Triton X-45{\textregistered} were suitable for extraction of haptoglobin (Hp) from fatty tissues. The extracted samples were subjected to electrophoresis and subsequently stained with o-tolidine. Enzyme-immunoassay and Western blotting (electrophoretic) techniques were used successfully to determine the Hp phenotypes from minute quantities of tissue samples after extractions with non-ionic detergents.",

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AB - Of the seven non-ionic detergents tested, Span 40®, Tween 20® and Triton X-45® were suitable for extraction of haptoglobin (Hp) from fatty tissues. The extracted samples were subjected to electrophoresis and subsequently stained with o-tolidine. Enzyme-immunoassay and Western blotting (electrophoretic) techniques were used successfully to determine the Hp phenotypes from minute quantities of tissue samples after extractions with non-ionic detergents.

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Haptoglobin typing from fatty tissues after extraction with non-ionic detergents

Abstract

All Science Journal Classification (ASJC) codes

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