TY - JOUR
T1 - Harnessing CRISPR-Cas systems for bacterial genome editing
AU - Selle, Kurt
AU - Barrangou, Rodolphe
N1 - Funding Information:
The authors thank all members of the CRISPR and TRK laboratory for constructive insights, especially Brant Johnson for reviewing the manuscript. This work was supported by start-up funds from North Carolina State University, a grant from the National Science Foundation (CBET-1403135), and the North Carolina Agriculture Foundation.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Manipulation of genomic sequences facilitates the identification and characterization of key genetic determinants in the investigation of biological processes. Genome editing via clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) constitutes a next-generation method for programmable and high-throughput functional genomics. CRISPR-Cas systems are readily reprogrammed to induce sequence-specific DNA breaks at target loci, resulting in fixed mutations via host-dependent DNA repair mechanisms. Although bacterial genome editing is a relatively unexplored and underrepresented application of CRISPR-Cas systems, recent studies provide valuable insights for the widespread future implementation of this technology. This review summarizes recent progress in bacterial genome editing and identifies fundamental genetic and phenotypic outcomes of CRISPR targeting in bacteria, in the context of tool development, genome homeostasis, and DNA repair.
AB - Manipulation of genomic sequences facilitates the identification and characterization of key genetic determinants in the investigation of biological processes. Genome editing via clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) constitutes a next-generation method for programmable and high-throughput functional genomics. CRISPR-Cas systems are readily reprogrammed to induce sequence-specific DNA breaks at target loci, resulting in fixed mutations via host-dependent DNA repair mechanisms. Although bacterial genome editing is a relatively unexplored and underrepresented application of CRISPR-Cas systems, recent studies provide valuable insights for the widespread future implementation of this technology. This review summarizes recent progress in bacterial genome editing and identifies fundamental genetic and phenotypic outcomes of CRISPR targeting in bacteria, in the context of tool development, genome homeostasis, and DNA repair.
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U2 - 10.1016/j.tim.2015.01.008
DO - 10.1016/j.tim.2015.01.008
M3 - Review article
C2 - 25698413
AN - SCOPUS:84925876620
SN - 0966-842X
VL - 23
SP - 225
EP - 232
JO - Trends in Microbiology
JF - Trends in Microbiology
IS - 4
ER -
