@article{516b0619d0be41f3ac019938fcff1ad7,
title = "Hemicellulose-Cellulose Composites Reveal Differences in Cellulose Organization after Dilute Acid Pretreatment",
abstract = " Model hemicellulose-cellulose composites that mimic plant cell wall polymer interactions were prepared by synthesizing deuterated bacterial cellulose in the presence of glucomannan or xyloglucan. Dilute acid pretreatment (DAP) of these materials was studied using small-angle neutron scattering, X-ray diffraction, and sum frequency generation spectroscopy. The macrofibril dimensions of the pretreated cellulose alone were smaller but with similar entanglement of macrofibrillar network as native cellulose. In addition, the crystallite size dimension along the (010) plane increased. Glucomannan-cellulose underwent similar changes to cellulose, except that the macrofibrillar network was more entangled after DAP. Conversely, in xyloglucan-cellulose the macrofibril dimensions and macrofibrillar network were relatively unchanged after pretreatment, but the cellulose I β content was increased. Our results point to a tight interaction of xyloglucan with microfibrils while glucomannan only interacts with macrofibril surfaces. This study provides insight into roles of different hemicellulose-cellulose interactions and may help in improving pretreatment processes or engineering plants with decreased recalcitrance.",
author = "Riddhi Shah and Shixin Huang and Pingali, {Sai Venkatesh} and Daisuke Sawada and Yunqiao Pu and Miguel Rodriguez and Ragauskas, {Arthur J.} and Kim, {Seong H.} and Evans, {Barbara R.} and Davison, {Brian H.} and Hugh O'Neill",
note = "Funding Information: R.S., D.S., S.V.P., H.O{\textquoteright}N., B.R.E., A.J.R, Y.P and B.H.D. acknowledge the support of the Genomic Science Program, Office of Biological and Environmental Research (OBER), U.S. Department of Energy, under Contract FWP ERKP752, for sample preparation for SANS and XRD studies. Biodeuteration and SANS studies on Bio-SANS were supported by the OBER funded Center for Structural Molecular Biology (CSMB) under Contract FWP ERKP291, using the High Flux Isotope Reactor supported by the Office of Basic Energy Sciences, U.S. Department of Energy. SFG studies performed by S.H and S.H.K were supported by the Center for Lignocellulose Structure and Formation, an Energy Frontier Research Center, funded by DOE Office of Basic Energy Sciences, under Award DE-SC0001090. We thank Kevin Weiss for technical assistance and Jill Hemman for graphic design. Part of the D2O used in this research was supplied by United States Department of Energy Office of Science by the Isotope Program in the office of Nuclear Physics. This manuscript has been authored by UT-Battelle, LLC under Contract DE-AC05-00OR22725 with the U.S. Department of Energy. Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",
year = "2019",
month = feb,
day = "11",
doi = "10.1021/acs.biomac.8b01511",
language = "English (US)",
volume = "20",
pages = "893--903",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "2",
}