TY - JOUR
T1 - Hepatitis B virus X protein differentially effects the ubiquitin-mediated proteasomal degradation of β-catenin depending on the status of cellular p53
AU - Jung, Jin Kyu
AU - Kwun, Hyun Jin
AU - Lee, Jung Ok
AU - Arora, Payal
AU - Jang, Kyung Lib
PY - 2007/8
Y1 - 2007/8
N2 - Abnormal accumulation of β-catenin is considered to be a strong driving force in hepatocellular carcinogenesis; however, the mechanism of β-catenin accumulation in tumours is unclear. Here, it was demonstrated that hepatitis B virus X protein (HBx) differentially regulates the level of β-catenin through two ubiquitin-dependent proteasome pathways depending on p53 status. In the presence of p53, HBx downregulated β-catenin through the activation of a p53-Siah-1 proteasome pathway. For this purpose, HBx upregulated Siah-1 expression at the transcriptional level via activation of p53. In the absence of p53, however, HBx stabilized β-catenin through the inhibition of a glycogen synthase kinase-3β-dependent pathway. Interestingly, HBx variants with a Pro-101 to Ser substitution were unable to activate p53 and thus could stabilize β-catenin irrespective of p53 status. Based on these findings, a model of β-catenin regulation by HBx is proposed whereby the balance between the two opposite activities of HBx determines the overall expression level of β-catenin. Differential regulation of β-catenin by HBx depending on host (p53 status) and viral factors (HBx sequence variation) helps not only to explain the observation that cancers accumulating β-catenin also exhibit a high frequency of p53 mutations but also to understand the contradictory reports on the roles of HBx during hepatocellular carcinogenesis.
AB - Abnormal accumulation of β-catenin is considered to be a strong driving force in hepatocellular carcinogenesis; however, the mechanism of β-catenin accumulation in tumours is unclear. Here, it was demonstrated that hepatitis B virus X protein (HBx) differentially regulates the level of β-catenin through two ubiquitin-dependent proteasome pathways depending on p53 status. In the presence of p53, HBx downregulated β-catenin through the activation of a p53-Siah-1 proteasome pathway. For this purpose, HBx upregulated Siah-1 expression at the transcriptional level via activation of p53. In the absence of p53, however, HBx stabilized β-catenin through the inhibition of a glycogen synthase kinase-3β-dependent pathway. Interestingly, HBx variants with a Pro-101 to Ser substitution were unable to activate p53 and thus could stabilize β-catenin irrespective of p53 status. Based on these findings, a model of β-catenin regulation by HBx is proposed whereby the balance between the two opposite activities of HBx determines the overall expression level of β-catenin. Differential regulation of β-catenin by HBx depending on host (p53 status) and viral factors (HBx sequence variation) helps not only to explain the observation that cancers accumulating β-catenin also exhibit a high frequency of p53 mutations but also to understand the contradictory reports on the roles of HBx during hepatocellular carcinogenesis.
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U2 - 10.1099/vir.0.82836-0
DO - 10.1099/vir.0.82836-0
M3 - Article
C2 - 17622616
AN - SCOPUS:34547600513
SN - 0022-1317
VL - 88
SP - 2144
EP - 2154
JO - Journal of General Virology
JF - Journal of General Virology
IS - 8
ER -