Heterologous expression of maize (Zea mays L.) Mir1 cysteine proteinase in eukaryotic and prokaryotic expression systems

T. Pechan; P. W.K. Ma; D. S. Luthe

doi:10.1016/j.pep.2003.11.024

Heterologous expression of maize (Zea mays L.) Mir1 cysteine proteinase in eukaryotic and prokaryotic expression systems

T. Pechan, P. W.K. Ma, D. S. Luthe

Plant Science

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Several heterologous expression systems were tested for their ability to express a unique maize cysteine proteinase Mir1. A baculovirus-based expression system using Trichoplusia ni larvae as host resulted in the expression of Mir1 that was correctly processed and exhibited proteinase activity. Expression in Escherichia coli resulted in accumulation of Mir1, but it had limited solubility and enzymatic activity. Large quantities of Mir1 were produced when Pichia pastoris was used as the host, but the enzyme was insoluble and inactive.

Original language	English (US)
Pages (from-to)	134-141
Number of pages	8
Journal	Protein Expression and Purification
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/j.pep.2003.11.024
State	Published - Mar 2004

All Science Journal Classification (ASJC) codes

Biotechnology

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10.1016/j.pep.2003.11.024

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abstract = "Several heterologous expression systems were tested for their ability to express a unique maize cysteine proteinase Mir1. A baculovirus-based expression system using Trichoplusia ni larvae as host resulted in the expression of Mir1 that was correctly processed and exhibited proteinase activity. Expression in Escherichia coli resulted in accumulation of Mir1, but it had limited solubility and enzymatic activity. Large quantities of Mir1 were produced when Pichia pastoris was used as the host, but the enzyme was insoluble and inactive.",

author = "T. Pechan and Ma, {P. W.K.} and Luthe, {D. S.}",

note = "Funding Information: We thank Dr. Wendell Roelofs (Cornell University) for supplying the Trichoplusia ni larvae used in this study and Dr. H. Alan Wood (Mississippi State University) for his guidance with the expression system. This research was supported by the Mississippi Agricultural and Forestry Experiment Station and the National Science Foundation (IBN-0236150 to D.S.L.). This article was approved for publication as No. J10402 of the Mississippi Agricultural and Forestry Experiment Station.",

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AU - Luthe, D. S.

N1 - Funding Information: We thank Dr. Wendell Roelofs (Cornell University) for supplying the Trichoplusia ni larvae used in this study and Dr. H. Alan Wood (Mississippi State University) for his guidance with the expression system. This research was supported by the Mississippi Agricultural and Forestry Experiment Station and the National Science Foundation (IBN-0236150 to D.S.L.). This article was approved for publication as No. J10402 of the Mississippi Agricultural and Forestry Experiment Station.

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Heterologous expression of maize (Zea mays L.) Mir1 cysteine proteinase in eukaryotic and prokaryotic expression systems

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