High-resolution dynamic mapping of histone-DNA interactions in a nucleosome

Michael A. Hall, Alla Shundrovsky, Lu Bai, Robert M. Fulbright, John T. Lis, Michelle D. Wang

Research output: Contribution to journalArticlepeer-review

311 Scopus citations


The nature of the nucleosomal barrier that regulates access to the underlying DNA during many cellular processes is not fully understood. Here we present a detailed map of histone-DNA interactions along the DNA sequence to near base pair accuracy by mechanically unzipping single molecules of DNA, each containing a single nucleosome. This interaction map revealed a distinct ∼5-bp periodicity that was enveloped by three broad regions of strong interactions, with the strongest occurring at the dyad and the other two about ∼40-bp from the dyad. Unzipping up to the dyad allowed recovery of a canonical nucleosome upon relaxation of the DNA, but unzipping beyond the dyad resulted in removal of the histone octamer from its initial DNA sequence. These findings have important implications for how RNA polymerase and other DNA-based enzymes may gain access to DNA associated with a nucleosome.

Original languageEnglish (US)
Pages (from-to)124-129
Number of pages6
JournalNature Structural and Molecular Biology
Issue number2
StatePublished - Feb 2009

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology


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