TY - JOUR
T1 - High-Resolution Gas Chromatographic/High-Resolution Mass Spectrometric Analysis of Human Adipose Tissue for 2,3,7,8-Tetrachlorodibenzo-p-dioxin
AU - Patterson, Donald G.
AU - Holler, James S.
AU - Lapeza, Chester R.
AU - Alexander, Louis R.
AU - Groce, Donald F.
AU - O'Connor, Ralph C.
AU - Smith, S. Jay
AU - Liddle, John A.
AU - Needham, Larry L.
PY - 1986/4
Y1 - 1986/4
N2 - An analytical method Is described for measuring 2,3,7,8-tetrachlorodlbenzo-p-dioxin (TCDD) and other TCDD isomers in human tissue samples using a highly specific cleanup procedure and high-resolution gas chromatography/hlgh-resolution mass spectrometry. The 2,3,7,8-TCDD Is quantified by the isotope dilution technique with [13C12]2,3,7,8-TCDD as the Internal standard. Other TCDD isomers are estimated by use of relative response factors. The 1 pad-per-trillion (pptr) limit of detection is adequate for determining 2,3,7,8-TCDD concentrations present In human adipose tissue specimens. Analytical accuracy Is demonstrated by the results obtained In analyzing several spiked samples. The method Is shown to be unaffected by a number of potentially interfering compounds. A series of quality control material is used to verify system performance. For samples containing 1.6 pptr 2,3,7,8-TCDD, a coefficient of variation (CV) of 19% is observed. For samples at the 8.4 pptr level, a CV of 11% Is observed.
AB - An analytical method Is described for measuring 2,3,7,8-tetrachlorodlbenzo-p-dioxin (TCDD) and other TCDD isomers in human tissue samples using a highly specific cleanup procedure and high-resolution gas chromatography/hlgh-resolution mass spectrometry. The 2,3,7,8-TCDD Is quantified by the isotope dilution technique with [13C12]2,3,7,8-TCDD as the Internal standard. Other TCDD isomers are estimated by use of relative response factors. The 1 pad-per-trillion (pptr) limit of detection is adequate for determining 2,3,7,8-TCDD concentrations present In human adipose tissue specimens. Analytical accuracy Is demonstrated by the results obtained In analyzing several spiked samples. The method Is shown to be unaffected by a number of potentially interfering compounds. A series of quality control material is used to verify system performance. For samples containing 1.6 pptr 2,3,7,8-TCDD, a coefficient of variation (CV) of 19% is observed. For samples at the 8.4 pptr level, a CV of 11% Is observed.
UR - http://www.scopus.com/inward/record.url?scp=0022578287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022578287&partnerID=8YFLogxK
U2 - 10.1021/ac00295a010
DO - 10.1021/ac00295a010
M3 - Article
C2 - 3706742
AN - SCOPUS:0022578287
SN - 0003-2700
VL - 58
SP - 705
EP - 713
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 4
ER -