TY - JOUR
T1 - High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis
AU - Laremore, Tatiana N.
AU - Ly, Mellisa
AU - Solakyildirim, Kemal
AU - Zagorevski, Dmitri V.
AU - Linhardt, Robert J.
N1 - Funding Information:
Financial support from National Institutes of Health Grants GM38060 , GM090127 , and HL096972 is gratefully acknowledged. The authors thank Dennis Pu and Samantha Cheong for their help in collecting SAX–HPLC fractions and preparation of Azure A assay.
PY - 2010/6
Y1 - 2010/6
N2 - Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.
AB - Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.
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U2 - 10.1016/j.ab.2010.03.004
DO - 10.1016/j.ab.2010.03.004
M3 - Article
C2 - 20211145
AN - SCOPUS:77952888996
SN - 0003-2697
VL - 401
SP - 236
EP - 241
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -