High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis

Tatiana N. Laremore; Mellisa Ly; Kemal Solakyildirim; Dmitri V. Zagorevski; Robert J. Linhardt

doi:10.1016/j.ab.2010.03.004

High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis

Tatiana N. Laremore
, Mellisa Ly
, Kemal Solakyildirim
, Dmitri V. Zagorevski
, Robert J. Linhardt

Huck Institutes of the Life Sciences

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.

Original language	English (US)
Pages (from-to)	236-241
Number of pages	6
Journal	Analytical Biochemistry
Volume	401
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2010.03.004
State	Published - Jun 2010

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2010.03.004

Cite this

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title = "High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis",

abstract = "Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.",

author = "Laremore, \{Tatiana N.\} and Mellisa Ly and Kemal Solakyildirim and Zagorevski, \{Dmitri V.\} and Linhardt, \{Robert J.\}",

note = "Funding Information: Financial support from National Institutes of Health Grants GM38060 , GM090127 , and HL096972 is gratefully acknowledged. The authors thank Dennis Pu and Samantha Cheong for their help in collecting SAX–HPLC fractions and preparation of Azure A assay. ",

year = "2010",

month = jun,

doi = "10.1016/j.ab.2010.03.004",

language = "English (US)",

volume = "401",

pages = "236--241",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis

AU - Laremore, Tatiana N.

AU - Ly, Mellisa

AU - Solakyildirim, Kemal

AU - Zagorevski, Dmitri V.

AU - Linhardt, Robert J.

N1 - Funding Information: Financial support from National Institutes of Health Grants GM38060 , GM090127 , and HL096972 is gratefully acknowledged. The authors thank Dennis Pu and Samantha Cheong for their help in collecting SAX–HPLC fractions and preparation of Azure A assay.

PY - 2010/6

Y1 - 2010/6

N2 - Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.

AB - Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.

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DO - 10.1016/j.ab.2010.03.004

M3 - Article

C2 - 20211145

AN - SCOPUS:77952888996

SN - 0003-2697

VL - 401

SP - 236

EP - 241

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis

Abstract

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