High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells

Xiaonan Yang; Zhuofa Chen; Gihoon Choi; Jun Miao; Liwang Cui; Weihua Guan

doi:10.1109/MEMSYS.2017.7863579

High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells

Xiaonan Yang, Zhuofa Chen, Gihoon Choi, Jun Miao, Liwang Cui, Weihua Guan

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This work reports a high throughput and label-free cell deformability microfluidic sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). As a mechanical biomarker, the RBC deformability is highly relevant to the infection status. The cell deformability is measured by evaluating the translocation time when each individual cell squeezes through a microscale constriction. More than 30,000 RBCs can be analyzed for parasitemia quantification in under 1 min with a throughput ∼500 cells/s. Moreover, the device can also differentiate various malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability. As compared to the microscopy and flow cytometry, this microfluidic deformability sensor would allow for label-free and rapid malaria parasitemia quantification and stage determination at a low-cost.

Original language	English (US)
Title of host publication	2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	997-1000
Number of pages	4
ISBN (Electronic)	9781509050789
DOIs	https://doi.org/10.1109/MEMSYS.2017.7863579
State	Published - Feb 23 2017
Event	30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States Duration: Jan 22 2017 → Jan 26 2017

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
Country/Territory	United States
City	Las Vegas
Period	1/22/17 → 1/26/17

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/MEMSYS.2017.7863579

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Yang, X., Chen, Z., Choi, G., Miao, J., Cui, L., & Guan, W. (2017). High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 (pp. 997-1000). Article 7863579 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2017.7863579

Yang, Xiaonan ; Chen, Zhuofa ; Choi, Gihoon et al. / High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 997-1000 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells",

abstract = "This work reports a high throughput and label-free cell deformability microfluidic sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). As a mechanical biomarker, the RBC deformability is highly relevant to the infection status. The cell deformability is measured by evaluating the translocation time when each individual cell squeezes through a microscale constriction. More than 30,000 RBCs can be analyzed for parasitemia quantification in under 1 min with a throughput ∼500 cells/s. Moreover, the device can also differentiate various malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability. As compared to the microscopy and flow cytometry, this microfluidic deformability sensor would allow for label-free and rapid malaria parasitemia quantification and stage determination at a low-cost.",

author = "Xiaonan Yang and Zhuofa Chen and Gihoon Choi and Jun Miao and Liwang Cui and Weihua Guan",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 ; Conference date: 22-01-2017 Through 26-01-2017",

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doi = "10.1109/MEMSYS.2017.7863579",

language = "English (US)",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

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Yang, X, Chen, Z, Choi, G, Miao, J, Cui, L & Guan, W 2017, High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. in 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017., 7863579, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 997-1000, 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017, Las Vegas, United States, 1/22/17. https://doi.org/10.1109/MEMSYS.2017.7863579

High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. / Yang, Xiaonan; Chen, Zhuofa; Choi, Gihoon et al.
2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 997-1000 7863579 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

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AU - Yang, Xiaonan

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AU - Cui, Liwang

AU - Guan, Weihua

PY - 2017/2/23

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N2 - This work reports a high throughput and label-free cell deformability microfluidic sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). As a mechanical biomarker, the RBC deformability is highly relevant to the infection status. The cell deformability is measured by evaluating the translocation time when each individual cell squeezes through a microscale constriction. More than 30,000 RBCs can be analyzed for parasitemia quantification in under 1 min with a throughput ∼500 cells/s. Moreover, the device can also differentiate various malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability. As compared to the microscopy and flow cytometry, this microfluidic deformability sensor would allow for label-free and rapid malaria parasitemia quantification and stage determination at a low-cost.

AB - This work reports a high throughput and label-free cell deformability microfluidic sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). As a mechanical biomarker, the RBC deformability is highly relevant to the infection status. The cell deformability is measured by evaluating the translocation time when each individual cell squeezes through a microscale constriction. More than 30,000 RBCs can be analyzed for parasitemia quantification in under 1 min with a throughput ∼500 cells/s. Moreover, the device can also differentiate various malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability. As compared to the microscopy and flow cytometry, this microfluidic deformability sensor would allow for label-free and rapid malaria parasitemia quantification and stage determination at a low-cost.

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ER -

Yang X, Chen Z, Choi G, Miao J, Cui L, Guan W. High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 997-1000. 7863579. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2017.7863579

High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells

Abstract

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All Science Journal Classification (ASJC) codes

UN SDGs

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