TY - GEN
T1 - High-throughput single molecule detection using nanophotonic/microfluidic hybrid chip
AU - Zhao, Yanhui
AU - Chen, Danqi
AU - Benkovic, Stephen J.
AU - Huang, Tony Jun
PY - 2014/1/1
Y1 - 2014/1/1
N2 - The ability of nanophotonic hole chips to guide light energy into subwavelength-diameter cylindrical nanoapertures has been exploited for single-molecule fluorescence studies of biomolecules at micromolar concentrations. Here, we present a nanophotonic/microfluidic hybrid chip for single-molecule fluorescence imaging. Our design allowed single-molecule FRET studies that revealed weak DNA-protein and protein-protein interactions found with T4 replisomal proteins.
AB - The ability of nanophotonic hole chips to guide light energy into subwavelength-diameter cylindrical nanoapertures has been exploited for single-molecule fluorescence studies of biomolecules at micromolar concentrations. Here, we present a nanophotonic/microfluidic hybrid chip for single-molecule fluorescence imaging. Our design allowed single-molecule FRET studies that revealed weak DNA-protein and protein-protein interactions found with T4 replisomal proteins.
UR - http://www.scopus.com/inward/record.url?scp=84941695862&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941695862&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84941695862
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 1811
EP - 1813
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -