Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

G. T. Slavov; G. T. Howe; I. Yakovlev; K. J. Edwards; K. V. Krutovskii; G. A. Tuskan; J. E. Carlson; S. H. Strauss; W. T. Adams

doi:10.1007/s00122-003-1490-y

Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

G. T. Slavov, G. T. Howe, I. Yakovlev, K. J. Edwards, K. V. Krutovskii, G. A. Tuskan, J. E. Carlson, S. H. Strauss, W. T. Adams

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Abstract

Twenty-two highly variable SSR markers were developed in Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.

Original language	English (US)
Pages (from-to)	873-880
Number of pages	8
Journal	Theoretical and Applied Genetics
Volume	108
Issue number	5
DOIs	https://doi.org/10.1007/s00122-003-1490-y
State	Published - Mar 2004

All Science Journal Classification (ASJC) codes

Biotechnology
Agronomy and Crop Science
Genetics

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title = "Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations",

abstract = "Twenty-two highly variable SSR markers were developed in Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.",

author = "Slavov, {G. T.} and Howe, {G. T.} and I. Yakovlev and Edwards, {K. J.} and Krutovskii, {K. V.} and Tuskan, {G. A.} and Carlson, {J. E.} and Strauss, {S. H.} and Adams, {W. T.}",

year = "2004",

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doi = "10.1007/s00122-003-1490-y",

language = "English (US)",

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T2 - Mendelian inheritance and map locations

AU - Slavov, G. T.

AU - Howe, G. T.

AU - Yakovlev, I.

AU - Edwards, K. J.

AU - Krutovskii, K. V.

AU - Tuskan, G. A.

AU - Carlson, J. E.

AU - Strauss, S. H.

AU - Adams, W. T.

PY - 2004/3

Y1 - 2004/3

N2 - Twenty-two highly variable SSR markers were developed in Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.

AB - Twenty-two highly variable SSR markers were developed in Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.

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JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

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ER -

Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

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