Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells

Thomas W. Gardner; Treena Lesher; Sonny Khin; Cuong Vu; Alistair J. Barber; William A. Brennan

doi:10.1042/bj3200717

Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells

Thomas W. Gardner, Treena Lesher, Sonny Khin, Cuong Vu, Alistair J. Barber, William A. Brennan

Research output: Contribution to journal › Article › peer-review

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Abstract

We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10^-9-10^-4 M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H₁ and H₂ receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.

Original language	English (US)
Pages (from-to)	717-721
Number of pages	5
Journal	Biochemical Journal
Volume	320
Issue number	3
DOIs	https://doi.org/10.1042/bj3200717
State	Published - Dec 15 1996

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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10.1042/bj3200717

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abstract = "We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10-9-10-4 M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H1 and H2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.",

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AU - Gardner, Thomas W.

AU - Lesher, Treena

AU - Khin, Sonny

AU - Vu, Cuong

AU - Barber, Alistair J.

AU - Brennan, William A.

PY - 1996/12/15

Y1 - 1996/12/15

N2 - We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10-9-10-4 M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H1 and H2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.

AB - We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10-9-10-4 M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H1 and H2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.

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Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells

Abstract

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