Spleen cells from A.TH mice, presensitized in vivo by skin grafting, were restimulated in vitro by A.TL lymphocytes, and A.TH anti-A.TL effector cells were generated. The effector cells lysed, in the CML assay, A.TL blasts. This reaction, which was against I-region antigens, could be inhibited by the addition to the reaction mixture of anti-La sera directed against A.TL antigens. The inhibition was specific, since normal mouse serum, reciprocal antiserum (A.TL anti-A.TH), and anti-H-2 sera did not have a significant effect on the reaction. The Ia antibodies also specifically inhibited the reaction of A.TH anti-A.TL effector cells against CBA targets. Con A blasts were significantly poorer targets in I-region CML than LPS blasts. As CML targets, macrophages and cells of a mammary adenocarcinoma were as good as, if not better than, the LPS blasts. The experiments support the notion that Ia antigens are the targets in the I-region CML.
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