TY - JOUR
T1 - Histone neighbors in nuclei and extended chromatin
AU - Hardison, Ross C.
AU - Zeitler, Deborah Parris
AU - Murphy, Joyce M.
AU - Chalkley, Roger
N1 - Funding Information:
We are grateful to Drs. Vaughn Jackson and Peter Hoffmann helpful discussions and advice, and to Dr. Steven Spiker advice on two-dimensional gels. This work was supported grants from the NIH.
PY - 1977/10
Y1 - 1977/10
N2 - Histone neighbors in compact and extended chromatin have been investigated by cross-linking histones in nuclei and in nucleohistone extended with 6 M urea, using the bifunctional reversible reagent methyl-4-mercaptobutyrimidate (MMB). Similar histone dimers are found in both conformational states of chromatin. The dimers most frequently found are H2b-H2a, H2b-H3 and H3-H2a; dimers found less frequently are H3-H4, H3-H3 and H2b-H4. More H3-H3 is found in nuclei than in extended chromatin. H1 is found predominantly as poly-H1, although it can be cross-linked to H2b or H3. After reaction with MMB, native compact chromatin is no longer extendable in 6 M urea, which shows that the reagent is capable of linking together histones holding the chromatin in a compact conformation. Thus the histone propinquity in extended chromatin mimics the intimate histone associations in compact chromatin.
AB - Histone neighbors in compact and extended chromatin have been investigated by cross-linking histones in nuclei and in nucleohistone extended with 6 M urea, using the bifunctional reversible reagent methyl-4-mercaptobutyrimidate (MMB). Similar histone dimers are found in both conformational states of chromatin. The dimers most frequently found are H2b-H2a, H2b-H3 and H3-H2a; dimers found less frequently are H3-H4, H3-H3 and H2b-H4. More H3-H3 is found in nuclei than in extended chromatin. H1 is found predominantly as poly-H1, although it can be cross-linked to H2b or H3. After reaction with MMB, native compact chromatin is no longer extendable in 6 M urea, which shows that the reagent is capable of linking together histones holding the chromatin in a compact conformation. Thus the histone propinquity in extended chromatin mimics the intimate histone associations in compact chromatin.
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U2 - 10.1016/0092-8674(77)90118-0
DO - 10.1016/0092-8674(77)90118-0
M3 - Article
C2 - 912751
AN - SCOPUS:0017699852
SN - 0092-8674
VL - 12
SP - 417
EP - 427
JO - Cell
JF - Cell
IS - 2
ER -