Histone neighbors in nuclei and extended chromatin

Ross C. Hardison; Deborah Parris Zeitler; Joyce M. Murphy; Roger Chalkley

doi:10.1016/0092-8674(77)90118-0

Histone neighbors in nuclei and extended chromatin

Ross C. Hardison
, Deborah Parris Zeitler
, Joyce M. Murphy
, Roger Chalkley

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Histone neighbors in compact and extended chromatin have been investigated by cross-linking histones in nuclei and in nucleohistone extended with 6 M urea, using the bifunctional reversible reagent methyl-4-mercaptobutyrimidate (MMB). Similar histone dimers are found in both conformational states of chromatin. The dimers most frequently found are H2b-H2a, H2b-H3 and H3-H2a; dimers found less frequently are H3-H4, H3-H3 and H2b-H4. More H3-H3 is found in nuclei than in extended chromatin. H1 is found predominantly as poly-H1, although it can be cross-linked to H2b or H3. After reaction with MMB, native compact chromatin is no longer extendable in 6 M urea, which shows that the reagent is capable of linking together histones holding the chromatin in a compact conformation. Thus the histone propinquity in extended chromatin mimics the intimate histone associations in compact chromatin.

Original language	English (US)
Pages (from-to)	417-427
Number of pages	11
Journal	Cell
Volume	12
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(77)90118-0
State	Published - Oct 1977

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/0092-8674(77)90118-0

Cite this

@article{79ce27a4604f4529a39459651495ab43,

title = "Histone neighbors in nuclei and extended chromatin",

abstract = "Histone neighbors in compact and extended chromatin have been investigated by cross-linking histones in nuclei and in nucleohistone extended with 6 M urea, using the bifunctional reversible reagent methyl-4-mercaptobutyrimidate (MMB). Similar histone dimers are found in both conformational states of chromatin. The dimers most frequently found are H2b-H2a, H2b-H3 and H3-H2a; dimers found less frequently are H3-H4, H3-H3 and H2b-H4. More H3-H3 is found in nuclei than in extended chromatin. H1 is found predominantly as poly-H1, although it can be cross-linked to H2b or H3. After reaction with MMB, native compact chromatin is no longer extendable in 6 M urea, which shows that the reagent is capable of linking together histones holding the chromatin in a compact conformation. Thus the histone propinquity in extended chromatin mimics the intimate histone associations in compact chromatin.",

author = "Hardison, \{Ross C.\} and Zeitler, \{Deborah Parris\} and Murphy, \{Joyce M.\} and Roger Chalkley",

note = "Funding Information: We are grateful to Drs. Vaughn Jackson and Peter Hoffmann helpful discussions and advice, and to Dr. Steven Spiker advice on two-dimensional gels. This work was supported grants from the NIH.",

year = "1977",

month = oct,

doi = "10.1016/0092-8674(77)90118-0",

language = "English (US)",

volume = "12",

pages = "417--427",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Histone neighbors in nuclei and extended chromatin

AU - Hardison, Ross C.

AU - Zeitler, Deborah Parris

AU - Murphy, Joyce M.

AU - Chalkley, Roger

N1 - Funding Information: We are grateful to Drs. Vaughn Jackson and Peter Hoffmann helpful discussions and advice, and to Dr. Steven Spiker advice on two-dimensional gels. This work was supported grants from the NIH.

PY - 1977/10

Y1 - 1977/10

N2 - Histone neighbors in compact and extended chromatin have been investigated by cross-linking histones in nuclei and in nucleohistone extended with 6 M urea, using the bifunctional reversible reagent methyl-4-mercaptobutyrimidate (MMB). Similar histone dimers are found in both conformational states of chromatin. The dimers most frequently found are H2b-H2a, H2b-H3 and H3-H2a; dimers found less frequently are H3-H4, H3-H3 and H2b-H4. More H3-H3 is found in nuclei than in extended chromatin. H1 is found predominantly as poly-H1, although it can be cross-linked to H2b or H3. After reaction with MMB, native compact chromatin is no longer extendable in 6 M urea, which shows that the reagent is capable of linking together histones holding the chromatin in a compact conformation. Thus the histone propinquity in extended chromatin mimics the intimate histone associations in compact chromatin.

AB - Histone neighbors in compact and extended chromatin have been investigated by cross-linking histones in nuclei and in nucleohistone extended with 6 M urea, using the bifunctional reversible reagent methyl-4-mercaptobutyrimidate (MMB). Similar histone dimers are found in both conformational states of chromatin. The dimers most frequently found are H2b-H2a, H2b-H3 and H3-H2a; dimers found less frequently are H3-H4, H3-H3 and H2b-H4. More H3-H3 is found in nuclei than in extended chromatin. H1 is found predominantly as poly-H1, although it can be cross-linked to H2b or H3. After reaction with MMB, native compact chromatin is no longer extendable in 6 M urea, which shows that the reagent is capable of linking together histones holding the chromatin in a compact conformation. Thus the histone propinquity in extended chromatin mimics the intimate histone associations in compact chromatin.

UR - https://www.scopus.com/pages/publications/0017699852

UR - https://www.scopus.com/pages/publications/0017699852#tab=citedBy

U2 - 10.1016/0092-8674(77)90118-0

DO - 10.1016/0092-8674(77)90118-0

M3 - Article

C2 - 912751

AN - SCOPUS:0017699852

SN - 0092-8674

VL - 12

SP - 417

EP - 427

JO - Cell

JF - Cell

IS - 2

ER -

Histone neighbors in nuclei and extended chromatin

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this