TY - JOUR
T1 - HMG-proteins 1 and 2 are required for transcription of chromatin by endogenous RNA polymerase
AU - Stoute, Jose A.
AU - Marzluff, William F.
N1 - Funding Information:
This research was supported by grant GM 27789 from the NIH. J.A.S. was supported by a NSF summer fellowship and a J. R. Fisher fellowship from the American Cancer Society, Florida Division and W.F.M. is a recipient of a Career Development Award CA 00178 from the NIH. We are grateful to Scott Chambers and Randolph Rill for a gift of crude mouse myeloma HMG proteins and for helpful discussions.
PY - 1982/8/31
Y1 - 1982/8/31
N2 - Chromatin prepared from isolated mouse myeloma cell nuclei faithfully transcribes RNA using endogenous RNA polymerase. Extraction of the chromatin with 0.35M KC1 greatly reduces the ability of the chromatin to transcribe RNA, but RNA synthesis is restored by adding back the extracted material. The major proteins extracted under these conditions are the high mobility group proteins - HMG 1 and 2. When purified HMG proteins 1 and 2 were added back to the extracted chromatin preparations total RNA synthesis was stimulated 3 to 5 fold. The synthesis of the RNA polymerase III products, 5S rRNA and tRNA precursors, was also stimulated the same amount.
AB - Chromatin prepared from isolated mouse myeloma cell nuclei faithfully transcribes RNA using endogenous RNA polymerase. Extraction of the chromatin with 0.35M KC1 greatly reduces the ability of the chromatin to transcribe RNA, but RNA synthesis is restored by adding back the extracted material. The major proteins extracted under these conditions are the high mobility group proteins - HMG 1 and 2. When purified HMG proteins 1 and 2 were added back to the extracted chromatin preparations total RNA synthesis was stimulated 3 to 5 fold. The synthesis of the RNA polymerase III products, 5S rRNA and tRNA precursors, was also stimulated the same amount.
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U2 - 10.1016/S0006-291X(82)80136-8
DO - 10.1016/S0006-291X(82)80136-8
M3 - Article
C2 - 6215919
AN - SCOPUS:0020494557
SN - 0006-291X
VL - 107
SP - 1279
EP - 1284
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -