Hollow fiber countercurrent dialysis for continuous buffer exchange of high-value biotherapeutics

Christopher J. Yehl, Mario G. Jabra, Andrew L. Zydney

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Buffer exchange, desalting, and formulation of high-value biotherapeutics are currently performed using batch diafiltration (DF); however, this type of tangential flow filtration process may be difficult to implement as part of a fully continuous biomanufacturing process. The objective of this study was to explore the potential of using countercurrent dialysis for continuous protein formulation and buffer exchange. Experiments were performed using concentrated solutions of immunoglobuin G (IgG) with commercially available hollow fiber dialyzers having 1.5 and 1.8 m 2 membrane surface area. More than 99.9% buffer exchange was obtained over a range of conditions, as determined from the removal of a model impurity (vitamin B 12 ). The dialyzers were able to process more than 0.5 kg of IgG per day in an easily scalable low-cost process. In addition, buffer requirements were less than 0.02 L of buffer per gram IgG, which is several times less than that used in current batch DF processes. These results clearly demonstrate the potential of using low-cost hollow fiber dialyzers for buffer exchange and product formulation in continuous bioprocessing.

Original languageEnglish (US)
Article numbere2763
JournalBiotechnology progress
Issue number2
StatePublished - Mar 1 2019

All Science Journal Classification (ASJC) codes

  • Biotechnology


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