Human adipose-derived stromal/stem cell isolation, culture, and osteogenic differentiation

Ammar T. Qureshi, Cong Chen, Forum Shah, Caasy Thomas-Porch, Jeffrey M. Gimble, Daniel J. Hayes

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations


Annually, more than 200,000 elective liposuction procedures are performed in the United States and over a million worldwide. The ease of harvest and abundance make human adipose-derived stromal/stem cells (hASCs) isolated from lipoaspirates an attractive, readily available source of adult stem cells that have become increasingly popular for use in many studies. Here, we describe common methods for hASC culture, preservation, and osteogenic differentiation. We introduce methods of ceramic, polymer, and composite scaffold synthesis with a description of morphological, chemical, and mechanical characterization techniques. Techniques for scaffold loading are compared, and methods for determining cell loading efficiency and proliferation are described. Finally, we provide both qualitative and quantitative techniques for in vitro assessment of hASC osteogenic differentiation.

Original languageEnglish (US)
Title of host publicationMethods of Adipose Tissue Biology, Part B
PublisherAcademic Press Inc.
Number of pages22
ISBN (Print)9780128002803
StatePublished - 2014

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology


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