Human cytosolic tRNase ZL can downregulate gene expression through miRNA

Reyad A. Elbarbary; Hiroaki Takaku; Naoto Uchiumi; Hiroko Tamiya; Mayumi Abe; Hiroshi Nishida; Masayuki Nashimoto

doi:10.1016/j.febslet.2009.09.015

Human cytosolic tRNase Z^L can downregulate gene expression through miRNA

Reyad A. Elbarbary
, Hiroaki Takaku
, Naoto Uchiumi
, Hiroko Tamiya
, Mayumi Abe
, Hiroshi Nishida
, Masayuki Nashimoto

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A long form of tRNase Z (tRNase Z^L) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase Z^L in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase Z^L. The present data suggest the possibility that human cytosolic tRNase Z^L modulates gene expression through a subset of microRNAs in the cells.

Original language	English (US)
Pages (from-to)	3241-3246
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2009.09.015
State	Published - Oct 6 2009

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2009.09.015

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title = "Human cytosolic tRNase ZL can downregulate gene expression through miRNA",

abstract = "A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.",

author = "Elbarbary, \{Reyad A.\} and Hiroaki Takaku and Naoto Uchiumi and Hiroko Tamiya and Mayumi Abe and Hiroshi Nishida and Masayuki Nashimoto",

note = "Funding Information: This paper is dedicated to Chikako Nashimoto, who worked for this project from the discovery of RNase 65 and deceased of multiple myeloma on 2 February, 2007. We thank Dr. Masamichi Takagi for helpful discussion and encouragement. This work was supported in part by the Science Research Promotion Fund and the Academic Frontier Research Project Grant from the Promotion and Mutual Aid Corporation for Private Schools of Japan . ",

year = "2009",

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doi = "10.1016/j.febslet.2009.09.015",

language = "English (US)",

volume = "583",

pages = "3241--3246",

journal = "FEBS Letters",

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T1 - Human cytosolic tRNase ZL can downregulate gene expression through miRNA

AU - Elbarbary, Reyad A.

AU - Takaku, Hiroaki

AU - Uchiumi, Naoto

AU - Tamiya, Hiroko

AU - Abe, Mayumi

AU - Nishida, Hiroshi

AU - Nashimoto, Masayuki

N1 - Funding Information: This paper is dedicated to Chikako Nashimoto, who worked for this project from the discovery of RNase 65 and deceased of multiple myeloma on 2 February, 2007. We thank Dr. Masamichi Takagi for helpful discussion and encouragement. This work was supported in part by the Science Research Promotion Fund and the Academic Frontier Research Project Grant from the Promotion and Mutual Aid Corporation for Private Schools of Japan .

PY - 2009/10/6

Y1 - 2009/10/6

N2 - A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.

AB - A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.

UR - https://www.scopus.com/pages/publications/70349456506

UR - https://www.scopus.com/pages/publications/70349456506#tab=citedBy

U2 - 10.1016/j.febslet.2009.09.015

DO - 10.1016/j.febslet.2009.09.015

M3 - Article

C2 - 19751732

AN - SCOPUS:70349456506

SN - 0014-5793

VL - 583

SP - 3241

EP - 3246

JO - FEBS Letters

JF - FEBS Letters

IS - 19

ER -

Human cytosolic tRNase Z^L can downregulate gene expression through miRNA

Abstract

All Science Journal Classification (ASJC) codes

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