Human immunodeficiency virus type 1 infection of H9 cells induces increased glucose transporter expression

Lynn R. Sorbara, Frank Maldarelli, Georges Chamoun, Brian Schilling, Sudhichai Chokekijcahi, Louis Staudt, Hiroaki Mitsuya, Ian A. Simpson, Steven L. Zeichner

    Research output: Contribution to journalArticlepeer-review

    71 Scopus citations

    Abstract

    A clone obtained from a differential display screen for cellular genes with altered expression during human immunodeficiency virus (HIV) infection matched the sequence for the human GLUT3 facilitative glucose transporter, a high-velocity-high-affinity facilitative transporter commonly expressed in neurons of the central nervous system. Northern (RNA) analysis showed that GLUT3 expression increased during infection. Flow cytometry showed that GLUT3 protein expression increased specifically in the HIV-infected cells; this increase correlated with increased 2-deoxyglucose transport in the HIV- infected culture. HIV infection therefore leads to increased expression of a glucose transporter normally expressed at high levels in other cell types and a corresponding increase in glucose transport activity. If HIV infection places increased metabolic demands on the host cell, changes in the expression of a cellular gene that plays an important role in cellular metabolism might provide a more favorable environment for vital replication.

    Original languageEnglish (US)
    Pages (from-to)7275-7279
    Number of pages5
    JournalJournal of virology
    Volume70
    Issue number10
    DOIs
    StatePublished - Oct 1996

    All Science Journal Classification (ASJC) codes

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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