Abstract
AIM: To analyse the humoral immune response in mice to nucleic acid vaccines (VR1012/HG-MSP1-17 for intracellular expression or VR1012/TPA/HG-MSP-17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT. METHODS: BALB/c or C57BL/6 mice received three times intramuscular immunization with 200 micrograms/100 microliters or 100 micrograms/100 microliters of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. Anti-HG or anti-MSP1-17 antibodies were monitored by indirect ELISA. RESULTS: BALB/c and C57BL/6 mice immunized with 100 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised significantly anti-HG and anti-MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/HG-MSP1-17 per mouse raised higher anti-HG antibodies but not anti-MSP1-17 antibodies. BALB/c mice immunized with 200 micrograms/100 microliters of VR1012/TPA/HG-MSP1-17 per mouse raised low level of anti-HG antibodies only. CONCLUSION: VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 302-304 |
| Number of pages | 3 |
| Journal | Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases |
| Volume | 17 |
| Issue number | 5 |
| State | Published - 1999 |
All Science Journal Classification (ASJC) codes
- General Medicine
