TY - JOUR
T1 - Hyaluronic acid and fibrin hydrogels with concentrated DNA/PEI polyplexes for local gene delivery
AU - Lei, Yuguo
AU - Rahim, Maha
AU - Ng, Quinn
AU - Segura, Tatiana
N1 - Funding Information:
The authors would like to thank the National Institute of Health ( R21EB007730 ) and the National Science Foundation (CAREER grant 0747539 ) for funding the research.
PY - 2011/8/10
Y1 - 2011/8/10
N2 - Local delivery of DNA through a hydrogel scaffold would increase the applicability of gene therapy in tissue regeneration and cancer therapy. However, the delivery of DNA/cationic polymer nanoparticles (polyplexes) using hydrogels is challenging due to the aggregation and inactivation of polyplexes during their incorporation into hydrogel scaffolds. We developed a novel process (termed caged nanoparticle encapsulation or CnE) to load concentrated and unaggregated non-viral gene delivery nanoparticles into various hydrogels. Previously, we showed that PEG hydrogels loaded with DNA/PEI polyplexes through this process were able to deliver genes both in vitro and in vivo. In this study, we found that hyaluronic acid and fibrin hydrogels with concentrated and unaggregated polyplexes loaded through CnE were able to deliver genes in vivo as well, demonstrating the universality of the process.
AB - Local delivery of DNA through a hydrogel scaffold would increase the applicability of gene therapy in tissue regeneration and cancer therapy. However, the delivery of DNA/cationic polymer nanoparticles (polyplexes) using hydrogels is challenging due to the aggregation and inactivation of polyplexes during their incorporation into hydrogel scaffolds. We developed a novel process (termed caged nanoparticle encapsulation or CnE) to load concentrated and unaggregated non-viral gene delivery nanoparticles into various hydrogels. Previously, we showed that PEG hydrogels loaded with DNA/PEI polyplexes through this process were able to deliver genes both in vitro and in vivo. In this study, we found that hyaluronic acid and fibrin hydrogels with concentrated and unaggregated polyplexes loaded through CnE were able to deliver genes in vivo as well, demonstrating the universality of the process.
UR - http://www.scopus.com/inward/record.url?scp=79960922709&partnerID=8YFLogxK
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U2 - 10.1016/j.jconrel.2011.01.028
DO - 10.1016/j.jconrel.2011.01.028
M3 - Article
C2 - 21295089
AN - SCOPUS:79960922709
SN - 0168-3659
VL - 153
SP - 255
EP - 261
JO - Journal of Controlled Release
JF - Journal of Controlled Release
IS - 3
ER -
