Hybrid adipose graft materials synthesized from chemically modified adipose extracellular matrix

Shue Li; Yiming Liu; Jacob McCann; Dino J. Ravnic; Jeffrey M. Gimble; Daniel J. Hayes

doi:10.1002/jbm.a.37273

Hybrid adipose graft materials synthesized from chemically modified adipose extracellular matrix

Shue Li, Yiming Liu, Jacob McCann, Dino J. Ravnic, Jeffrey M. Gimble, Daniel J. Hayes

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Decellularized extracellular matrix (ECM) from tissues is a promising biomaterial that can provide a complex 3D microenvironment capable of modulating cell response and tissue regeneration. In this study, we have integrated the decellularized thiolated adipose-derived ECM, at different concentrations, with polyethylene glycol (PEG) using Michael addition between thiol and acrylate moieties. The potential for this material to support adipogenic differentiation of human adipose-derived stem cells was evaluated by encapsulating cells in hydrogels with increasing concentrations of chemically modified ECM (mECM). Our results demonstrated a positive correlation between the ECM content in the hydrogels and cell proliferation, adipogenic marker expression, and lipid formation and accumulation. Furthermore, we have shown host cell infiltration and enhanced adipogenesis in vivo after implantation. These findings support the graft as a potential alternative for adipose tissue regeneration.

Original language	English (US)
Pages (from-to)	156-163
Number of pages	8
Journal	Journal of Biomedical Materials Research - Part A
Volume	110
Issue number	1
DOIs	https://doi.org/10.1002/jbm.a.37273
State	Published - Jan 2022

All Science Journal Classification (ASJC) codes

Ceramics and Composites
Biomaterials
Biomedical Engineering
Metals and Alloys

Access to Document

10.1002/jbm.a.37273

Cite this

@article{dcd8fd901cbe42d4861097bd0591c10b,

title = "Hybrid adipose graft materials synthesized from chemically modified adipose extracellular matrix",

abstract = "Decellularized extracellular matrix (ECM) from tissues is a promising biomaterial that can provide a complex 3D microenvironment capable of modulating cell response and tissue regeneration. In this study, we have integrated the decellularized thiolated adipose-derived ECM, at different concentrations, with polyethylene glycol (PEG) using Michael addition between thiol and acrylate moieties. The potential for this material to support adipogenic differentiation of human adipose-derived stem cells was evaluated by encapsulating cells in hydrogels with increasing concentrations of chemically modified ECM (mECM). Our results demonstrated a positive correlation between the ECM content in the hydrogels and cell proliferation, adipogenic marker expression, and lipid formation and accumulation. Furthermore, we have shown host cell infiltration and enhanced adipogenesis in vivo after implantation. These findings support the graft as a potential alternative for adipose tissue regeneration.",

author = "Shue Li and Yiming Liu and Jacob McCann and Ravnic, {Dino J.} and Gimble, {Jeffrey M.} and Hayes, {Daniel J.}",

note = "Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC.",

year = "2022",

month = jan,

doi = "10.1002/jbm.a.37273",

language = "English (US)",

volume = "110",

pages = "156--163",

journal = "Journal of Biomedical Materials Research - Part A",

issn = "1549-3296",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - Hybrid adipose graft materials synthesized from chemically modified adipose extracellular matrix

AU - Li, Shue

AU - Liu, Yiming

AU - McCann, Jacob

AU - Ravnic, Dino J.

AU - Gimble, Jeffrey M.

AU - Hayes, Daniel J.

PY - 2022/1

Y1 - 2022/1

N2 - Decellularized extracellular matrix (ECM) from tissues is a promising biomaterial that can provide a complex 3D microenvironment capable of modulating cell response and tissue regeneration. In this study, we have integrated the decellularized thiolated adipose-derived ECM, at different concentrations, with polyethylene glycol (PEG) using Michael addition between thiol and acrylate moieties. The potential for this material to support adipogenic differentiation of human adipose-derived stem cells was evaluated by encapsulating cells in hydrogels with increasing concentrations of chemically modified ECM (mECM). Our results demonstrated a positive correlation between the ECM content in the hydrogels and cell proliferation, adipogenic marker expression, and lipid formation and accumulation. Furthermore, we have shown host cell infiltration and enhanced adipogenesis in vivo after implantation. These findings support the graft as a potential alternative for adipose tissue regeneration.

AB - Decellularized extracellular matrix (ECM) from tissues is a promising biomaterial that can provide a complex 3D microenvironment capable of modulating cell response and tissue regeneration. In this study, we have integrated the decellularized thiolated adipose-derived ECM, at different concentrations, with polyethylene glycol (PEG) using Michael addition between thiol and acrylate moieties. The potential for this material to support adipogenic differentiation of human adipose-derived stem cells was evaluated by encapsulating cells in hydrogels with increasing concentrations of chemically modified ECM (mECM). Our results demonstrated a positive correlation between the ECM content in the hydrogels and cell proliferation, adipogenic marker expression, and lipid formation and accumulation. Furthermore, we have shown host cell infiltration and enhanced adipogenesis in vivo after implantation. These findings support the graft as a potential alternative for adipose tissue regeneration.

UR - http://www.scopus.com/inward/record.url?scp=85110131983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85110131983&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.37273

DO - 10.1002/jbm.a.37273

M3 - Article

C2 - 34263999

AN - SCOPUS:85110131983

SN - 1549-3296

VL - 110

SP - 156

EP - 163

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

IS - 1

ER -

Hybrid adipose graft materials synthesized from chemically modified adipose extracellular matrix

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this