TY - JOUR
T1 - Hydrogen-deuterium exchange mass spectrometry (HDX-MS) centroid data measured between 3.6 oC and 25.4 OC for the Fab fragment of NiStmab
AU - Hudgens, Jeffrey W.
AU - Gallagher, Elyssia S.
AU - Karageorgos, Ioannis
AU - Anderson, Kyle W.
AU - Huang, Richard Y.C.
AU - Chen, Guodong
AU - Bou-Assaf, George M.
AU - Espada, Alfonso
AU - Chalmers, Michael J.
AU - Harguindey, Eduardo
AU - Zhang, Hui Min
AU - Walters, Benjamin T.
AU - Zhang, Jennifer
AU - Venable, John
AU - Steckler, Caitlin
AU - Park, Inhee
AU - Brock, Ansgar
AU - Lu, Xiaojun
AU - Pandey, Ratnesh
AU - Chandramohan, Arun
AU - Anand, Ganesh Srinivasan
AU - Nirudodhi, Sasidhar N.
AU - Sperry, Justin B.
AU - Rouse, Jason C.
AU - Carroll, James A.
AU - Rand, Kasper D.
AU - Leurs, Ulrike
AU - Weis, David D.
AU - Al-Naqshabandi, Mohammed A.
AU - Hageman, Tyler S.
AU - Deredge, Daniel
AU - Wintrode, Patrick L.
AU - Papanastasiou, Malvina
AU - Lambris, John D.
AU - Li, Sheng
AU - Urata, Sarah
N1 - Publisher Copyright:
© 2019 National Institute of Standards and Technology. All rights reserved.
PY - 2019/5/2
Y1 - 2019/5/2
N2 - The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the Fab Fragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 oC and 25.4 oC for seven intervals ranging over (0 to 14,400) s plus a ∞pseudo s control. The deuterium content of peptic peptide fragments were measured by mass spectrometry. [1-3] These data were reported by fifteen laboratories, which conducted the measurements using orbitrap and Q-TOF mass spectrometers. The cohort reported ≈ 78,900 centroids for 430 proteolytic peptide sequences of the heavy and light chains of NISTmAb, providing nearly 100 % coverage. In addition, some groups reported ≈ 10,900 centroid measurements for 77 peptide sequences of the Fc fragment. The instrumentation and physical and chemical conditions under which these data were acquired are documented.
AB - The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the Fab Fragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 oC and 25.4 oC for seven intervals ranging over (0 to 14,400) s plus a ∞pseudo s control. The deuterium content of peptic peptide fragments were measured by mass spectrometry. [1-3] These data were reported by fifteen laboratories, which conducted the measurements using orbitrap and Q-TOF mass spectrometers. The cohort reported ≈ 78,900 centroids for 430 proteolytic peptide sequences of the heavy and light chains of NISTmAb, providing nearly 100 % coverage. In addition, some groups reported ≈ 10,900 centroid measurements for 77 peptide sequences of the Fc fragment. The instrumentation and physical and chemical conditions under which these data were acquired are documented.
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U2 - 10.6028/jres.124.009
DO - 10.6028/jres.124.009
M3 - Article
AN - SCOPUS:85066796565
SN - 1044-677X
VL - 124
JO - Journal of Research of the National Institute of Standards and Technology
JF - Journal of Research of the National Institute of Standards and Technology
M1 - 124009
ER -