TY - JOUR
T1 - Identification and amplification of microsatellite loci in deep-sea tubeworms of the genus Escarpia (Polychaeta, Siboglinidae)
AU - Cowart, Dominique A.
AU - Huang, Chunya
AU - Schaeffer, Stephen W.
N1 - Funding Information:
National Science Foundation (Award #1209688) and the Alfred P. Sloan Scholarship. Any opinions, findings, conclusions, or recommendations expressed in this article are those of the authors and do not necessarily reflect the views of the National Science Foundation.
PY - 2013/6
Y1 - 2013/6
N2 - Deep-sea cold seeps support diverse communities that include vestimentiferan tubeworms of the genus Escarpia. To investigate population structure to understand how deep-sea communities are established, several polymorphic microsatellites were developed using 454 pyrosequencing. Eleven and sixteen loci were identified in Escarpia laminata (n = 129) and Escarpia southwardae (n = 80). Additionally, the amplification of microsatellites is expected to be successful in closely related species and can help resolve their phylogenetic relationships. Nine of the E. laminata loci were amplified across three Escarpia species (n = 229). Four of the twenty-eight loci deviated from Hardy-Weinburg Equilibrium and no linkage disequilibrium was found in the loci tested here.
AB - Deep-sea cold seeps support diverse communities that include vestimentiferan tubeworms of the genus Escarpia. To investigate population structure to understand how deep-sea communities are established, several polymorphic microsatellites were developed using 454 pyrosequencing. Eleven and sixteen loci were identified in Escarpia laminata (n = 129) and Escarpia southwardae (n = 80). Additionally, the amplification of microsatellites is expected to be successful in closely related species and can help resolve their phylogenetic relationships. Nine of the E. laminata loci were amplified across three Escarpia species (n = 229). Four of the twenty-eight loci deviated from Hardy-Weinburg Equilibrium and no linkage disequilibrium was found in the loci tested here.
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U2 - 10.1007/s12686-012-9832-1
DO - 10.1007/s12686-012-9832-1
M3 - Article
AN - SCOPUS:84876822018
SN - 1877-7252
VL - 5
SP - 479
EP - 482
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -