Gangliosides containing 350 μg of sialic acids were isolated from 2.85 x 1011 rat erythrocytes and found to be mainly composed of GDla and an unknown alkali-labile species which was converted to GD la after treatment with ammonia. Smaller amounts of GM1 and Fuc-GMl were also present. Identification of the sialic acids of the novel species by thin-layer chromatography, high performance liquid chromatography and gas-liquid chromatography - mass spectrometry revealed the presence of both N- acetylneuraminic acid and N-acetyl-9-O-acetyl-neuraminic acid in about equimolar amounts. Incubation of the isolated ganglioside with Vibrio cholerae sialidase released N-acetyl-9-O-acetyl-neuraminic acid. Non O-acetylated Gm i was identified as the only remaining ganglioside by thin-layer chromatography. Thus this novel ganglioside has the following structure: Neu5,9Ac2α2-3Galβ1-3GalNAcβ4(Neu5Acα-2-3)Galβ14Glcβ1-l'Cer.
|Number of pages
|Hoppe-Seyler's Zeitschrift fur Physiologische Chemie
|Published - 1984
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