Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)

A. Lehner; Michael A. Campbell; N. C. Wheeler; T. Pöykkö; J. Glössl; J. Kreike; D. B. Neale

doi:10.1007/BF00223924

Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)

A. Lehner, Michael A. Campbell, N. C. Wheeler, T. Pöykkö, J. Glössl, J. Kreike, D. B. Neale

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The pendula phenotype of Norway spruce [Picea abies (L.) Karst f. pendula] is characterized by narrow crowns and strong apical dominance and is controlled by a single dominant gene (P). This defined genetic control presents one of the few opportunities to map a single gene controlling a morphological trait in a forest tree. We used random amplified polymorphic DNA (RAPD) markers and bulked segregant analysis to identify one locus OPH10_720, linked to the pendula gene. The estimated recombination frequency (r) between OPH10_720 and P was 0.046 (SE_r=0.032). Mapping of the pendula gene is an important first step towards the ultimate identification and cloning of this gene.

Original language	English (US)
Pages (from-to)	1092-1094
Number of pages	3
Journal	Theoretical and Applied Genetics
Volume	91
Issue number	6-7
DOIs	https://doi.org/10.1007/BF00223924
State	Published - Nov 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Agronomy and Crop Science
Genetics

Access to Document

10.1007/BF00223924

Cite this

@article{5515c786a8ab4f899cb78fee4aab64f1,

title = "Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)",

abstract = "The pendula phenotype of Norway spruce [Picea abies (L.) Karst f. pendula] is characterized by narrow crowns and strong apical dominance and is controlled by a single dominant gene (P). This defined genetic control presents one of the few opportunities to map a single gene controlling a morphological trait in a forest tree. We used random amplified polymorphic DNA (RAPD) markers and bulked segregant analysis to identify one locus OPH10_720, linked to the pendula gene. The estimated recombination frequency (r) between OPH10_720 and P was 0.046 (SEr=0.032). Mapping of the pendula gene is an important first step towards the ultimate identification and cloning of this gene.",

author = "A. Lehner and Campbell, {Michael A.} and Wheeler, {N. C.} and T. P{\"o}ykk{\"o} and J. Gl{\"o}ssl and J. Kreike and Neale, {D. B.}",

year = "1995",

month = nov,

doi = "10.1007/BF00223924",

language = "English (US)",

volume = "91",

pages = "1092--1094",

journal = "Theoretical and Applied Genetics",

issn = "0040-5752",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "6-7",

}

TY - JOUR

T1 - Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)

AU - Lehner, A.

AU - Campbell, Michael A.

AU - Wheeler, N. C.

AU - Pöykkö, T.

AU - Glössl, J.

AU - Kreike, J.

AU - Neale, D. B.

PY - 1995/11

Y1 - 1995/11

N2 - The pendula phenotype of Norway spruce [Picea abies (L.) Karst f. pendula] is characterized by narrow crowns and strong apical dominance and is controlled by a single dominant gene (P). This defined genetic control presents one of the few opportunities to map a single gene controlling a morphological trait in a forest tree. We used random amplified polymorphic DNA (RAPD) markers and bulked segregant analysis to identify one locus OPH10_720, linked to the pendula gene. The estimated recombination frequency (r) between OPH10_720 and P was 0.046 (SEr=0.032). Mapping of the pendula gene is an important first step towards the ultimate identification and cloning of this gene.

AB - The pendula phenotype of Norway spruce [Picea abies (L.) Karst f. pendula] is characterized by narrow crowns and strong apical dominance and is controlled by a single dominant gene (P). This defined genetic control presents one of the few opportunities to map a single gene controlling a morphological trait in a forest tree. We used random amplified polymorphic DNA (RAPD) markers and bulked segregant analysis to identify one locus OPH10_720, linked to the pendula gene. The estimated recombination frequency (r) between OPH10_720 and P was 0.046 (SEr=0.032). Mapping of the pendula gene is an important first step towards the ultimate identification and cloning of this gene.

UR - http://www.scopus.com/inward/record.url?scp=0001377201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001377201&partnerID=8YFLogxK

U2 - 10.1007/BF00223924

DO - 10.1007/BF00223924

M3 - Article

C2 - 24170001

AN - SCOPUS:0001377201

SN - 0040-5752

VL - 91

SP - 1092

EP - 1094

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

IS - 6-7

ER -

Identification of a RAPD marker linked to the pendula gene in Norway spruce (Picea abies (L.) Karst. f. pendula)

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this